High-level expression of porcine teschovirus 3D protein gene in Escherichia coli and analysis of its biological activity;

Expression of porcine teschovirus Swine/CH/IMH/03 strain VP1 protein in Escherichia coli and analysis of its reactinogenicity

Porcine teschovirus(PTV)was described as causative agents of severe and mild neurological disorders known as Teschen/Talfan disease,which callsed reproductive failure,pneumonia,diarrhea,and dermal lesions of swine.

Porcine teschovirus (PTV) was described as causative agents of severe and mild neurological disorders known as Teschen/Talfan disease, reproductive failure, pneumonia, diarrhea, and dermal lesions of swine.

Expression and Immunological Activity of E2 Epitope of Classical swine fever virus;

Pathogenicity of classical swine fever virus GX-3/98 strain and immunization relativity with attenuated hogcholera virus Chinese C strain;

Kinetics of peripheral blood leukocytes apoptosis and changes of CD4~+ and CD8~+ T lymphocyte subpopulations of pigs infected with classical swine fever virus;

The recombinant retroviral vector pBABE-puro-E2 was constructed by inserting classical swine fever virus(CSFV) Shimen strain E2 gene into pBABE-puro.

A classical swine fever virus(CSFV) E2 gene was codon-optimized according to the Sf9 cell codon usage.

A truncated gene encoding the major antigenic domains of E2 protein of classical swine fever virus(CSFV) was amplified by RT-PCR from the genomic RNA of CSFV C-strain and cloned into pPROEX-HTb expression vector to obtain recombinant pPROEX-tE2.

Fusion expression of multiepitope gene of CSFV envelope glycoprotein E2 and its protection efficiency against homologous challenge in rabbits;

Prediction,synthesis and characteristics of B-cell epitopes of CSFV envelope glycoprotein E2;

Detection of the Interference to the Reproduction of CSFV by PK-15 Cell Strains Transcribing shRNA Targeted to the 3′-UTR;