1) mispairing PCR-RFLP
错配PCR-RFLP
1.
A mispairing PCR-RFLP technique was applied in this study to determine the Insulin-like Growth Factor 2(IGF2) gene intron3 G3072A mutation in an outbred Landrace and Large White, and the gelded boars from Landrace× Large White cross.
采用错配PCR-RFLP技术鉴别了长白猪和大白猪纯种以及长白×大白猪杂交后代去势公猪胰岛素样生长因子2(IGF2)基因内含子3的3072位点变异,分析了遗传来自父本不同等位基因个体间的相关性状差异及不同等位基因的遗传效应。
2) PCR RFLP
PCR-RFLP
1.
Twenty two Bradyrhizobium peanut strains isolated from the root nodules of peanut growing at four different sites in Sichuan province,southwest China,were characterized by intrinsic antibiotic resistance (IAR),randomly amplified polymorphic DNAs(RAPDs)and 16S 23S rDNA PCR RFLP to investigate the genomic diversity.
用IAR、RAPDs和16S-23SPCR-RFLP3种方法对采集自四川省4个不同地点的22株慢生型花生根瘤菌的遗传多样性进行了研究。
3) PCR-RFLP and PCR
PCR-RFLP及PCR
4) mismatch PCR
错配PCR
1.
To further validate this SNP(single-nucleotide polymorphism) locus,the technique of mismatch PCR was established and amplified to examine the samples of embryo,paternal and maternal genomic DNA.
利用DNA测序技术,在荷斯坦奶牛胎儿中找到X染色体基因GLRA2 3’端的杂合位点(C/T),设计错配引物在该位点对胎儿及其父本、母本基因组进行PCR扩增,建立起GLRA2基因的错配PCR技术,该技术可以快速在群体基因组中检测这一杂合位点。
5) mismatch-PCR
错配PCR方法
1.
The results showed that the mismatch-PCR method developed for gyrA and parC mutations may be a simple,specific and rapid method for detection of this important fluoroquinolone resistance mutation and provide a useful tool for clinical diagnosis or epidemiological studies.
根据耐药沙门氏菌基因突变位点碱基变化情况,设计合适的引物进行错配PCR,鉴别突变位点,使其结果与测序结果相吻合,以建立错配PCR方法快速检测耐氟喹诺酮类药物沙门氏菌基因突变,判断菌株是否耐药。
6) PCR-RFLP
PCR-RFLP方法
1.
Objective: To evaluate TaqMan-MGB Probes genotyping method feasibility in detecting a well-known SNP, compared with PCR-RFLP.
目的:评估TaqMan-MGB探针基因分型方法检测已知SNP的可行性,并与传统的PCR-RFLP方法比较。
补充资料：密码错配
分子式：
CAS号：
性质： 在DNA的全长上存在的非互补的碱基，而本来应该是碱基对。
CAS号：
性质： 在DNA的全长上存在的非互补的碱基，而本来应该是碱基对。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条