1) suppression subtractive hybridization
抑制性削减杂交
1.
To discover genes differently expressed between lactation periods,suppression subtractive hybridization method was employed to construct a cDNA library expressed in mammary gland of 60th but not or little in 28th.
为了研究不同泌乳期乳腺组织中差异表达基因,利用抑制性削减杂交技术构建西农萨能羊泌乳60 d乳腺组织及泌乳28 d乳腺组织中差异表达基因文库,用Real-Time PCR技术验证阳性克隆血清淀粉样蛋白A 3(SAA3)基因,通过RT-PCR方法克隆西农萨能羊乳腺组织SAA3,并进行序列比对和功能预测。
2.
To study genes involved in human T cell signal transduction, a suppression subtractive hybridization (SSH) technique was developed to isolate the cDNA clones of genes that contained specific (differentially expressed) transcrips of T cells activated with anti-CD3 and anti-CD28 serving as“tester”, and the no.
为了研究参与人T细胞信号转导的基因,本文以正常人初始T细胞作为对照组,以抗人CD3单抗联合抗人CD28单抗激发的人T细胞作为实验组,进行抑制性削减杂交。
2) Suppression subtractive hybridization
抑制削减杂交
3) suppression subtractive hybridization
抑制性消减杂交
1.
Screening and cloning of target genes transactivated by human gene C1 encoding protein 1 interacting with HBV core antigen using suppression subtractive hybridization technique;
抑制性消减杂交技术筛选乙型肝炎病毒核心蛋白相互作用蛋白编码基因C1的反式调节基因
2.
Construction of cDNA library of hepatitis B virus with X protein C-terminally truncated 40 amino acids by suppression subtractive hybridization method;
抑制性消减杂交构建乙型肝炎病毒X蛋白羧基端缺失40个氨基酸cDNA文库
3.
Identification of genes associated with the metastasis of adenoid cystic carcinoma by suppression subtractive hybridization;
应用抑制性消减杂交技术分析涎腺腺样囊性癌转移相关基因
4) suppression subtractive hybridization
抑制性差减杂交
1.
Suppression subtractive hybridization(SSHs) was performed to distinguish the differentially expressed genes.
取培养48h的2组细胞提取总RNA后分离mRNA,应用抑制性差减杂交法分离差异表达基因,并连接于pGEM TEasyVector,转化大肠杆菌JM109,构成差减cDNA文库,经蓝白斑筛选后挑选白色菌落,提取质粒,序列测定后进行同源性比较分析。
2.
A systematic study was initiated to identify differentially expressed genes in the process of sex reversal of the grouper by using suppression subtractive hybridization (SSH) technique.
应用抑制性差减杂交 (SSH)技术构建了石斑鱼性反转前后性腺组织的SMARTcDNA文库及其cDNA差减文库 ,从中随机挑取 12 0 0个克隆进行了PCR和斑点杂交筛选 ,得到 12 0个差异表达cDNA片段 。
3.
Objective: To Construct a subtracted cDNA library of hyperlipidemia sensitive rabbits′liver by suppression subtractive hybridization(SSH).
结论:用抑制性差减杂交及T/A克隆技术成功构建了高脂血症敏感兔差异表达基因差减cDNA文库。
5) SSH
抑制性消减杂交
1.
Screening Correlative Genes from Citrinin Biosynthetic Pathway in Monascus Species of by SSH;
应用抑制性消减杂交法筛选与红曲菌产桔霉素相关的基因
2.
Methods:Suppression Subtractive Hybridization(SSH) was performed to profile differentially express genes between normal abortive fetus (as the driver) and Down s syndrome fetus(as the tester),then screening and analyzing the subtractive libraries.
方法:利用抑制性消减杂交的方法对DS胎儿及正常胎儿脑组织样品进行正向消减杂交,利用斑点杂交对所获的基因片断进行进一步的验证。
3.
coli132(UPEC132)by suppression subtractive hybridization(SSH).
目的:应用抑制性消减杂交技术,构建致肾盂肾炎大肠埃希菌132株(UPEC132)与已完成基因组测序的非致病性大肠埃希菌标准株MG1655的基因组DNA消减文库。
6) suppression subtractive hybridization(SSH)
抑制性消减杂交
1.
METHODS The functional genes differentially expressed in JAR cell line treated with cyclosporin A were screened by means of suppression subtractive hybridization(SSH).
方法应用抑制性消减杂交筛选1。
2.
A full-length cDNA of class glutathione S-transferase gene,designated as HvGSTF,was screened from Haynaldia villosa cDNA Library by EST639,a differentially expressed fragment isolated by suppression subtractive hybridization(SSH).
利用抑制性消减杂交(SSH)方法克隆差异表达片段EST639,从簇毛麦cDNA文库中筛选到一个类谷胱甘肽硫转移酶基因(GST)的全长cDNA,命名为HvGSTF。
补充资料：抑制杂交翻译
分子式：
CAS号：
性质：又称杂交扣留翻译。能使一克隆DNA与它所编码的蛋白质相关联的方法。这个方法基于下述事实：以RNA在与它的DNA互补体杂交后就不会在无细胞的翻译系统中指导蛋白质的合成。无细胞系统所合成的蛋白质，包括一个特定的杂交mRNA，就能与未发生同DNA杂交的系统所合成的蛋白质相比较，其差别就表示了由所研究的mRNA编码的蛋白质的性质。
CAS号：
性质：又称杂交扣留翻译。能使一克隆DNA与它所编码的蛋白质相关联的方法。这个方法基于下述事实：以RNA在与它的DNA互补体杂交后就不会在无细胞的翻译系统中指导蛋白质的合成。无细胞系统所合成的蛋白质，包括一个特定的杂交mRNA，就能与未发生同DNA杂交的系统所合成的蛋白质相比较，其差别就表示了由所研究的mRNA编码的蛋白质的性质。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条