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1)  real-time quantitative PCR(qPCR)
实时定量PCR(qPCR)
2)  Real time-amplification refractory mutation system-quantitative PCR
实时定量PCR技术和扩增阻滞突变(RT-ARMS-qPCR)系统
3)  realtime PCR/RT-PCR
实时定量PCR/RT-PCR
4)  real-time quantitative PCR
实时定量PCR
1.
Real-time quantitative PCR in determination of telomere length;
荧光实时定量PCR检测端粒长度
2.
Detection of IgH rearrangements using real-time quantitative PCR and Its Reaction Parameters;
IgH重排的实时定量PCR检测及反应参数研究
3.
Principles of real-time quantitative PCR and its application in plant pathology;
实时定量PCR的原理及其在植物病理学研究中的应用
5)  Quantitative real-time PCR
实时定量PCR
1.
287 Gy·min-1;total dose:7 Gy) exposuring,6 months later they were killed,then total RNA and total protein were extracted from thymic lymphomas and normal thymus tissue and cDNA was synthesized,the expressions of c-myc mRNA and protein in thymic lymphomas induced by ionizing radiation and normal thymus were detected by quantitative real-time PCR and Western blotting respectively.
min-1,总剂量7Gy)照射BALB/c小鼠,建立小鼠胸腺淋巴瘤模型,6个月后处死小鼠,取辐射诱发小鼠胸腺淋巴瘤和正常胸腺,分别提取总RNA、合成cDNA和提取总蛋白,采用实时定量PCR方法和Western blotting方法分别检测辐射诱发胸腺淋巴瘤和正常胸腺组织c-myc基因mRNA和蛋白表达。
2.
The design principles and application in medicine ,molecular biology, food testing of the quantitative real-time PCR were reviewed in this paper.
笔者对实时定量PCR试验设计原则及其在医学、分子生物学、食品检测的应用上进行了概述。
6)  RT-PCR
实时定量PCR
1.
Establishment of RT-PCR detecting relative expression of sBD-1-mRNA in Mongolian sheep
蒙古绵羊β-防御素基因相对表达水平实时定量PCR方法的建立
2.
Method:IGF-IR mRNA expression in marrow cells from patients with MDS and normal controls was quantitatively determined by real time reverse transcript polymerase chain reaction(real time RT-PCR).
方法:用实时定量PCR(real time RT-PCR)方法检测胰岛素样生长因子-I受体mRNA的表达量。
补充资料:immune PCR
分子式:
CAS号:

性质: 通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上,从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上,进而证明有抗原存在。目前最为敏感的检测方法,理论上可测得一个抗原分子的存在。

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