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1)  RT-PCR
RT-PC
1.
Development and Clinical Application of RT-PCR Differential Diagnosis Method for High Virulent Porcine Reproductive and Respiratory Syndrome;
高致病性猪繁殖与呼吸综合征病RT-PCR快速鉴别诊断方法的建立与临床应用
2)  R t
Rt值
3)  reverse transcription-polymerase chain reaction
RT-PCR
1.
With beta-actin gene as the internal standard,reverse transcription-polymerase chain reaction(RT-PCR) was used to examine the expression of fms-like tyrosine kinase(Flt-1) mRNA in endometrium of Small Tail Han sheep and Northeast Fine-wool sheep.
以β-actin基因作为内源性内标,采用RT-PCR方法检测了Flt-1基因mRNA在东北细毛羊、小尾寒羊妊娠期不同阶段子宫内膜中的表达量。
2.
MethodsThe expression of FEZ1 mRNA in 50 cases of esophageal carcinoma and 50 cases of normal esophageal mucoma were detected by reverse transcription-polymerase chain reaction(RT-PCR).
方法采用RT-PCR法检测50例食管鳞癌及50例正常食管组织中FEZ1 mRNA的表达情况,分析其与食管鳞癌患者各临床病理指标的关系。
3.
Methods The mRNA of Ki-67 and VEGF were detected using reverse transcription-polymerase chain reaction(RT-PCR) in 55 RCC tissues and 20 normal renal tissues.
方法应用半定量RT-PCR技术检测55例肾癌组织和20例正常肾组织Ki-67和VEGFmRNA表达情况。
4)  Reverse transcription polymerase chain reaction
RT-PCR
1.
Then the level of VEGF mRNA in kidney was determined by reverse transcription polymerase chain reaction (RT-PCR); the expression of VEGF and VEGF.
【方法】取3月龄、12月龄大鼠作为对照组,24月龄大鼠作为观察组各6只,提取其肾脏组织的RNA,应用逆转录聚合酶链反应技术(RT-PCR)检测肾脏组织内VEGFmRNA转录水平,以及应用免疫组化技术检测肾小球VEGF和VEGFR蛋白的表达;应用酶联免疫法检测肾组织培养液的VEGF总含量。
2.
Methods Reverse transcription polymerase chain reaction (RT PCR) was used to evaluate the amount of insulin like growth factor mRNA in exercise induced damaged skeletal muscle, and specimens were used for ultrastructural observation.
方法利用IGF引物对不同损伤程度骨骼肌标本进行RT-PCR检测,同时观查损伤骨骼肌超微结构改变。
3.
Methods NIT-1 cells were exposed to cyclosporin A (10 μmol/L) for 24 and 48 h respectively, after which the amount of insulin release was determined by means of radioimmunoassay (RIA), and the differential expressions of Nuox23, Cox7c and Atp5K genes assessed by semi-quantitative reverse transcription polymerase chain reaction.
放射免疫测定法(RIA)检测胰岛素释放,半定量RT-PCR检测CsA处理NIT-1胰岛β细胞后线粒体氧化磷酸化酶系中的几种主要成员(Nuox23、Cox7c和Atp5K)在mRNA水平上的表达。
5)  RT domain
RT域
6)  RTPCR
RT-PCR
1.
Expression of cbfa1 gene in rat osteosarcoma UMR106 cell was detected by RTPCR after the 72?h s treatment of FSK88 liquid with different mol concentration(25,50,100?μmol/L),Retinoic Acid(RA,10?μmol/L) and mixed liquid of FSK88 and RA(1∶1).
以大鼠成骨肉瘤细胞———UMR106为模型,应用RT-PCR方法,检测用不同浓度的FSK88药液(25,50,100μmol/L)、维甲酸(RA,10μmol/L,阳性对照)以及FSK88+RA(等体积)混合药液处理细胞72 h后,细胞内cbfa1基因表达量的变化。
2.
by RTPCR and 3′-RACE technology.
]实生苗为材料,采用RT-PCR及RACE技术,获得了PPF-1(开花、衰老相关基因)同源基因的cDNA全长序列PtPPF-1,该cDNA全长1 493 bp,编码452个氨基酸,与豌豆PPF-1、拟南芥ALB3、水稻PPF-1及葡萄一未命名基因的氨基酸序列同源性较高,说明PPF-1基因在进化过程中可能变异较小。
补充资料:PC/AAS blend PC/ABS
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性质:物性相似,但老化性能明显改善,长期老化后色泽和强度变化小。韧性好,以具有美丽的珍珠光泽和金属光泽而引人注目,用此种共混物生产的珠光塑料制品不像加入珠光颜料的塑料制品那样具有毒性,所以特别适于制取食品和化妆品的容器或作装饰品。耐热老化性、耐沸水性及耐应力开裂性均比单纯PC高。各生产厂产品性能不同,以Geloy为例,有通用型、高冲击型、高光泽型等品级。高冲击型的拉伸强度46MPa,弯曲模量2100MPa,悬臂梁缺口冲击强度95~106J/m;通用型冲击强度为54J/m,热变形温度(1.82MPa)88℃。用机械共混法制备。可注塑加工各种制品,用于户外耐老化的汽车部件和工业制件。

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