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1)  freezing-thawing repeatedly
反复冷冻融化
2)  repeated freezing and thawing
反复冻融
1.
Conclusion:Ultrasonic method combined with repeated freezing and thawing .
结论:超声波法结合反复冻融可有效提取云芝胞内胃蛋白酶抑制剂。
3)  freezing and thawing
反复冻融
1.
Results:The cell broken efficiency could reach 90% by ultrasonic method and reach 50% by freezing and thawing repetitive method.
方法采用超声波、反复冻融2种破壁方法,分别对三种不同密度的小球藻进行了破碎研究,并研究了不同破壁方法对小球藻海藻油提取率的影响。
2.
After repeatedly freezing and thawing 8 times between modified Frey medium and mycoplamas medium,or modified Frey medium at 37℃ for 24 hours and mycoplamas medium at 37℃ for 8 hours,the sensitivity of medium retained invariability.
改良Frey培养基与支原体培养基经反复冻融8次;或改良Frey培养基经37℃保存24h;支原体培养基37℃保存8h均不影响灵敏度。
4)  freeze-thawing
冷冻-融化
5)  repeated freeze-thaw method
反复冻融法
1.
In order to study the best conditions of the Sevag method for removing the proteins in Astragalus polysaccharide,and also to compare the Sevag method with repeated freeze-thaw method on protein removing,the single-factor experiment was conducted to study the effects of different ratios of solvent volume,dosages,and treating time on protein removing.
为研究Sevag法除黄芪粗多糖中蛋白质的最佳条件,并比较Sevag法和反复冻融法的除蛋白质效果,采用单因素试验方法,研究不同溶剂比例、用量、处理时间对蛋白质去除的影响。
6)  freeze/thaw cycle
反复冷冻-解冻
1.
The hydrogel membranes of PVA/Sericin were prepared by freeze/thaw cycles.
采用反复冷冻-解冻的方法制备了聚乙烯醇/丝胶共混凝胶薄膜,考察了薄膜热性能、力学性能和在水中的失重率,并对其微观结构进行了分析。
补充资料:冷冻-融化
分子式:
CAS号:

性质:破碎细胞的一种方法。将细胞在低温下突然冷冻(-15℃)和室温下融化,反复多次而达到破壁作用。由于冷冻,既能使细胞膜的疏水键结构破裂,从而增加了细胞的亲水性能,又因胞内水结晶,使细胞内外溶液浓度变化,引起细胞突然膨胀而破裂,本法适用于细胞壁较脆弱的菌体。

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