Objective:To investigate the effects of long-term administration of morphine on preprodynorphin mRNA (PPD mRNA) levels in different brain sites of rats, including hypothalamus, hippocampus and striatum.

By using intrathecal administration of antisense oligodeoxynucleotide (AS-ODN) against preprodynorphin mRNA in rats, we observed that this treatment could block both the formalin-induced behavioral nociceptive responses and the increased expression of dynorphin A (1-8) in the dorsal horn, with the increased expression of c-Fos protein being unchanged.

And In situ hybridization results indicated centipede abstract could prevent the high-level expression of the proenkephalin mRNA It suggested that the high-l.

) application antisense oligodeoxynucleotide (AS-ODN) to induce prodynorphin (Pro-Dyn) mRNA and observed the degree of inflammatory edema after the formalin injection in rats to prove whether the quantity of dynorphin A in nerve centre could effect the degree of periphery inflammation.

Total RNA in hippocampus was extracted and primers of enkaphalin or prodynorphin were respectively added for RT-PCR reaction.

Aim: To investigate the changes of enkaphalin mRNA and prodynorphin mRNA gene expression in rat brain regions with chro-(nic) immobilization stress and the influence of Chinese herbs.