1) duplex polymerase chain reaction
双重聚合酶链反应
1.
Methods Sputum samples from patients with clinically diagnosed legionella pneumonia according to manifestations and serum antibody results were detected for legionella DNA with a duplex polymerase chain reaction(DPCR)method.
方法采用双重聚合酶链反应(DPCR)法对根据临床表现及血清抗体结果,临床诊断为军团菌肺炎患者的痰标本进行军团菌DNA检测。
2) Pla-F1-PCR
双重式聚合酶链反应
3) multiplex polymerase chain reaction
多重聚合酶链反应
1.
Identification of Brucella by multiplex polymerase chain reaction;
布鲁氏菌多重聚合酶链反应鉴定研究
2.
Objective To apply a rapid and simple multiplex polymerase chain reaction(PCR) for detecting blaTEM,blaSHV and blaOXA-1 genes in Enterobacteriaceae.
目的应用多重聚合酶链反应(PCR)法在肠杆菌科细菌中检测blaTEM、blaSHV、blaOXA-1基因。
4) multiple PCR
多重聚合酶链反应
1.
AIM: To develop a simple,economical,accurate and practical method for the detection of Mycoplasma pneumoniae (MP) and Chlamydia pneumoniae (CP) using Template-directed dye-terminator incorporation with fluorescence polarization (TDI-FP) and a multiple PCR.
目的:应用多重聚合酶链反应和荧光偏振检测技术建立一种方便可靠、简单经济的肺炎支原体、肺炎衣原体DNA同步检测方法。
2.
Methods:Multiple PCR was used to detect Y chromosome microdeletion in AZFa,AZFb and AZFc on 103 cases of idiopathic azoospermia,72 cases of severe idiopathic oligozoospermia,and 60 cases of healthy male controls.
方法:采用多重聚合酶链反应技术对103例原发无精子症、72例原发严重少精症患者及60例正常生育男性进行AZFa、AZFb、AZFc3个区域微缺失分析。
5) multiple polymerase chain reaction
多重聚合酶链反应
1.
Detection of methicillin resistant staphylococcus aureus using multiple polymerase chain reaction;
耐甲氧西林金黄色葡萄球菌的多重聚合酶链反应检测方法
2.
Objective To evaluate the reliability and clinical practicability of minimal inhibitory concentration (MIC), cefoxitin disk diffusion test, multiple polymerase chain reaction(PCR) amplification of the mecA gene for detection of methicillin-resistant Staphylococci(MRS).
方法按2004年美国临床实验室标准化委员会推荐的头孢西丁纸片扩散(K-B)法、琼脂二倍稀释(MIC)法,从中筛选出表型耐甲氧西林葡萄球菌(MRS),再将受试的葡萄球菌进行多重聚合酶链反应的体外扩增检测mecA基因。
6) Multiplex real-time polymerase chain reaction
多重实时聚合酶链反应
补充资料：聚合酶链反应
聚合酶链反应
polymerase chain reaction,PCR
是用特异性寡核苷酸引物在DNA聚合酶的作用下对靶DNA序列进行大量扩增的分子生物学方法。PCR技术中以在短时间内把极其微量的特定DNA(或RNA）片断大量增扩，达到用常规方法就可以检测的水平。临床用来确定一些疾病的病原体，因其假阳性率较高，故应谨慎使用。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条