1) Map-based cloning
图位克隆
1.
Morphological Research of Rice Root Hair Mutants by Cryo-SEM and Map-based Cloning of Mutant Genes;
水稻根毛突变体Cryo-SEM观察及突变基因的图位克隆
2.
The high-density markers are necessary for map-based cloning of rice genes,but the available marker could not reach the satisfying level.
在水稻功能基因的图位克隆中,常需要高密度的分子标记,而目前公布的各种标记的密度还远未达到令人满意的程度。
3.
Using map-based cloning technique, OsALB23 has been mapped to a region of 280 kb between molecular markers R2M501 and R2M502on chromosome 2 (Fig.
利用图位克隆的方法,我们将基因定位于水稻第二条染色体分子标记R2M501和R2M502之间约280kb范围内。
2) map based cloning
图位克隆
1.
The gene cloning technigues include map based cloning or positional cloning,transposon or T DNA tagging method,homology based candidate gene method,expressed sequence tagging method and some differentially expressed gene cloning method in this study.
综述了基因克隆常用技术包括图位克隆法、转座子或T DNA标签法、同源序列法、表达序列标签(EST)法和差异表达基因分离方法的原理、应用、应用的潜力和限制因
2.
This paper summarized the progresses of the map based cloning and described the strategies in which should be adopted to clone a gene in rice.
图位克隆是基因分离的有效方法。
3) positional cloning
图位克隆
1.
One gene(FAC11) involves in zygotic activation has been rough mapped by positional cloning and another gene(FAC14) has been fine mapped, also two genes have been cloned(FAC2 and FAC17), subsequently one gene(FAC6) has been functionally elucidated in details.
2、利用图位克隆的方法,对其中的1个突变基因进行了粗定位(FAC11),又对其中的1个突变基因进行了精细定位(FAC14),2个突变体进行了基因的克隆(FAC2和FAC17),其中又对一个突变体(FAC6)进行了较深入的基因功能研究。
4) map-based cloing
图为克隆
5) Positional cloning
定位克隆
1.
This method thus has been successfully applied toperform positional cloning and to construct detailed transcription maps.
该法已成功地应用于定位克隆和详尽的转录图谱的构建。
2.
One strategy of locatingtumor suppressor genes and cloning these genes is to analyse the high rates of LOH in humanmalignant tumors in positional cloning of tumor suppressor genes.
在定位克隆抑癌基因的过程中，对肿瘤中高频率染色体杂合性丢失的分析是对抑癌基因进行定位并最终发现和克隆该类基因的先决条件之一。
3.
Gene cloning comprise two types of cloning methods: positional cloning and phenotype cloning.
基因克隆一般分为定位克隆和表型克隆。
6) cloning site
克隆位点
补充资料：多克隆位点
分子式：
CAS号：
性质：在所使用的载体插入外源片段的位置，加上一段人工合成的寡聚脱氧核糖核酸(ONT)，这段ONT由一连串的预设的限制性内切酶切点组成，每一切点之间的碱基数是3的倍数，这样插入不同酶切位点都不会引起推移。
CAS号：
性质：在所使用的载体插入外源片段的位置，加上一段人工合成的寡聚脱氧核糖核酸(ONT)，这段ONT由一连串的预设的限制性内切酶切点组成，每一切点之间的碱基数是3的倍数，这样插入不同酶切位点都不会引起推移。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条