2) suppression subtractive hybridization (SSH)
抑制性扣除杂交(SSH)
1.
Identification of Nitrate-induced genes by Suppression Subtractive Hybridization (SSH) and Their Expression Pattern Analysis in Rice Root;
利用抑制性扣除杂交（SSH）技术克隆水稻根系硝态氮诱导基因及诱导基因表达谱的研究
3) suppression subtractive hybridization
抑制扣除杂交
1.
To identify differentially expressed genes between fetal mesenchymal stem cell (MSC) and adult MSC, especially specified genes expressed in fetal MSC, a cDNA subtractive library of fetal MSC was constructed using suppression subtractive hybridization (SSH) technique.
为了获得胎儿和成人间充质干细胞 (mesenchymalstemcells,MSC)的差异表达基因 ,尤其是在胎儿MSC中特异表达的基因 ,应用抑制扣除杂交技术构建了胎儿和成人MSCcDNA扣除文库。
4) subtractive hybridization
扣除杂交
1.
Separation of gene clones related to crocin synthesis using subtractive hybridization;
用扣除杂交法分离藏红花苷合成相关基因的克隆
2.
Methods The differentially expressed genes were cloned by modified PCR-based subtractive hybridization.
方法利用聚合酶链反应改良扣除杂交技术,克隆正常组与腭裂组小鼠腭突组织中差异表达的基因,经反向点杂交鉴定后挑选阳性克隆并测序及同源性分析,Northern印迹杂交检测所获的新基因mRNA的全长。
3.
The technique,principle,advantage and disadvantage of several mRNA diffrential display means, such as differential screening,subtractive hybridization,reveise transcription PCR, representational difference analysis,suppression subtractive hybridization and its application in rice molecular biology.
对mRNA差异显示法中人们比较熟悉的示差筛选、扣除杂交和DDRT PCR法 ,以及近几年新提出的RDA、SSH 5种主要的差异基因筛选法 ,从其原理、在分子生物学中的应用、优缺点及其改进方法等方面进行了简要概
5) suppression subtractive hybridization
抑制性消减杂交
1.
Screening and cloning of target genes transactivated by human gene C1 encoding protein 1 interacting with HBV core antigen using suppression subtractive hybridization technique;
抑制性消减杂交技术筛选乙型肝炎病毒核心蛋白相互作用蛋白编码基因C1的反式调节基因
2.
Construction of cDNA library of hepatitis B virus with X protein C-terminally truncated 40 amino acids by suppression subtractive hybridization method;
抑制性消减杂交构建乙型肝炎病毒X蛋白羧基端缺失40个氨基酸cDNA文库
3.
Identification of genes associated with the metastasis of adenoid cystic carcinoma by suppression subtractive hybridization;
应用抑制性消减杂交技术分析涎腺腺样囊性癌转移相关基因
6) suppression subtractive hybridization
抑制性差减杂交
1.
Suppression subtractive hybridization(SSHs) was performed to distinguish the differentially expressed genes.
取培养48h的2组细胞提取总RNA后分离mRNA,应用抑制性差减杂交法分离差异表达基因,并连接于pGEM TEasyVector,转化大肠杆菌JM109,构成差减cDNA文库,经蓝白斑筛选后挑选白色菌落,提取质粒,序列测定后进行同源性比较分析。
2.
A systematic study was initiated to identify differentially expressed genes in the process of sex reversal of the grouper by using suppression subtractive hybridization (SSH) technique.
应用抑制性差减杂交 (SSH)技术构建了石斑鱼性反转前后性腺组织的SMARTcDNA文库及其cDNA差减文库 ,从中随机挑取 12 0 0个克隆进行了PCR和斑点杂交筛选 ,得到 12 0个差异表达cDNA片段 。
3.
Objective: To Construct a subtracted cDNA library of hyperlipidemia sensitive rabbits′liver by suppression subtractive hybridization(SSH).
结论:用抑制性差减杂交及T/A克隆技术成功构建了高脂血症敏感兔差异表达基因差减cDNA文库。
补充资料：抑制杂交翻译
分子式：
CAS号：
性质：又称杂交扣留翻译。能使一克隆DNA与它所编码的蛋白质相关联的方法。这个方法基于下述事实：以RNA在与它的DNA互补体杂交后就不会在无细胞的翻译系统中指导蛋白质的合成。无细胞系统所合成的蛋白质，包括一个特定的杂交mRNA，就能与未发生同DNA杂交的系统所合成的蛋白质相比较，其差别就表示了由所研究的mRNA编码的蛋白质的性质。
CAS号：
性质：又称杂交扣留翻译。能使一克隆DNA与它所编码的蛋白质相关联的方法。这个方法基于下述事实：以RNA在与它的DNA互补体杂交后就不会在无细胞的翻译系统中指导蛋白质的合成。无细胞系统所合成的蛋白质，包括一个特定的杂交mRNA，就能与未发生同DNA杂交的系统所合成的蛋白质相比较，其差别就表示了由所研究的mRNA编码的蛋白质的性质。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条