1) two dimentional DNA electrophoresis
二维DNA电泳
1.
Methods hMLH1 mutation was measured by two dimentional DNA electrophoresis and DNA sequencing;MSI was analyzed by PCR based methods.
方法 采用二维DNA电泳和DNA测序技术检测hMLH1突变 ;采用PCR为基础的方法检测MSI。
2) two-dimensional electrophoresis
二维电泳
1.
Optimized Two-dimensional Electrophoresis for Proteomic Analysis of Retinal Pigment Epithelium Cells;
视网膜色素上皮细胞蛋白质组学研究中二维电泳条件的优化
2.
Differential proteomic analysis for the visual cortex of young and adult Long Evans rat by two-dimensional electrophoresis;
成年与幼年Long Evans大鼠视皮层蛋白的二维电泳分析
3.
And proteomics technology based on two-dimensional electrophoresis is an effective approach to discover the changes in the states of protein phosphorylation.
以二维电泳为基础的蛋白质组学是发现蛋白磷酸化状态改变的有效途径。
3) 2-DE
二维电泳
1.
yoelii were separated with two-dimensional polyacrylamide gel electrophoresis(2-DE) respectively.
方法以大劣按蚊-约氏疟原虫为实验模型,利用二维电泳技术分离和比较饱吸约氏疟原虫感染的鼠血和正常鼠血后24 h的大劣按蚊血淋巴蛋白,获得差异蛋白,并对差异蛋白进行N端氨基酸测序和生物信息学分析,从而推测差异蛋白的功能。
2.
Objective: To separate the low abundance protein and establish the 2-DE synthetic map of total protein of human normal spermatozoa by using the 2-DE technology.
目的:运用二维电泳(2-DE)技术分离人类正常精子低丰度蛋白并建立全精子蛋白的2-DE整合图。
3.
Introduction: Established the method of prepare human seminal plasmaprotein for 2-DE and the 2-DE map of board range pH 3-10 and thenarrower range pH 4-7 immobilized gradient strip (IPG, 24 cm) in ourinstitute.
研究目的： 在本所建立和优化人类精浆蛋白提取方法，建立较窄pH梯度(pH4-7)二维电泳图谱和液化各个时间点宽pH梯度(pH3-10)二维电泳图谱。
6) two-dimensional gel electrophoresis
二维电泳
1.
Objective To analyze the protein related with ocular dominance plasticity termination in visual cortical area 17 of Long Evans rats by two-dimensional gel electrophoresis and mass spectrometry,and discuss the application of proteome technology in the study of visual plasticity.
目的利用二维电泳-质谱技术分析LongEvans大鼠初级视皮层中与眼优势柱终止相关的蛋白质,探索蛋白质组学技术在视觉可塑性研究中的应用。
2.
Two-dimensional gel electrophoresis(2-DE) profiles of normal and infarcted cardiac muscle tissues were compared;over 800 protein spots were separated in the silver-stained gels with the pH range of 5～8.
建立了大鼠急性心肌梗死模型,利用二维电泳(2DE)分离正常与梗死心肌组织的蛋白,得到不同条件下的蛋白质组图谱。
3.
The IEF protocols of the two-dimensional gel electrophoresis for bovine spermatozoa proteins was optimizied,the staining methods were comparied and the 2 D image was analyzied with the software of Image Master 6.
以牛精子全蛋白为试验材料进行二维电泳试验研究,优化和改进了一向等电聚焦参数,比较了不同的染色方法,并运用Image Master 6。
补充资料：Sds-聚丙烯酰胺凝胶电泳凝胶电泳
sds-聚丙烯酰胺凝胶电泳（sds-page，sodium dodecyl sulfate-polyacrylamide gel electrohoresis）
在有去污剂十二烷基硫酸钠存在下的聚丙烯酰胺凝胶电泳。sds-page只是按照分子大小分离的，而不是根据分子所带的电荷和大小分离的。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条