Using promoter probe vector pCB182, three different nifH:: lacZ transcriptional fusion plasmids were constructed, (B-galactosidase activity was detected in vivo in Escherichia coli, in the presence or absence of NifA of Kleb-siella pneumoniae, respectively, the results demonstrated that; 1.

However, some sporadic studies indicate that transcription can sometimes read-through the intergenic region and generates a large fusion transcript which contains the upstream gene, intergenic region and the downstream adjace.

Methods Primers and TaqMan probes were designed for detecting various fusion transcripts,and normal ABL gene was used as the internal contro1.

To investigate the biological features of leukemic cells in bcr/abl fusion transcript positive B lineage acute lymphoblastic leukemia (B ALL), 3 or 4 color flow cytometry with directly conjugated monoclonal antibodies was used to detect the immunophenotype of the cells in 26 patients with bcr/able positive B ALL and 32 patients with bcr/abl negative B ALL.