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1)  Dot-enzyme immunoassay
斑点酶免疫试验
2)  Dot-ELISA
斑点-酶联免疫吸附试验
3)  Dot-ELISA
斑点酶联免疫吸附试验
1.
Checking Salmonella in Animal Feed by Dot-ELISA;
应用斑点酶联免疫吸附试验快速检测饲料中的沙门氏菌
2.
[Objective]To detect the anti-42000 protein from egg nucleus antibody in systemic lupus erythematosus(SLE)patients with Dot Enzyme linked immunosorbent assay(Dot-ELISA),so as to explore the relationship between the anti-42000 protein from egg nucleus antibody and the correlative antibodies in SLE patients and to study it s role in the course of disease occurrence.
[目的]采用斑点酶联免疫吸附试验(Dot-ELISA)法检测系统性红斑狼疮(SLE)患者血清中抗鸡卵核42000蛋白的抗体,探讨该抗体与SLE患者相关抗体检测指标的关系,以进一步研究该抗体在SLE患者发病过程中的作用。
3.
The McAb can also identify CEF infected by MDV through dot-ELISA.
1∶ 10 0稀释时与 GA株感染的 CEF在斑点酶联免疫吸附试验 (dot- EL ISA)中呈阳性。
4)  dot blot enzyme immunoassays(DB-EIA)
斑点酶标记免疫试验
1.
Using RSD-infected seedcanes of 2 sugarcane varieties,YT00-236 and ROC22,as materials,the control effect of hot water treatment(HWT) at 50℃ for 2 hours on RSD was studied,combined with dot blot enzyme immunoassays(DB-EIA) to detect causal bacterium of RSD,Leifsonia xyli subsp.
应用50℃2 h热水处理严重感染RSD的粤糖00-236,新台糖22号种茎,用斑点酶标记免疫试验(Dot blot en-zyme immunoassays,DB-EIA)检测RSD病原菌(Leifsonia xylisubsp。
5)  dot ELISA
斑点酶联免疫吸附试验
1.
3 strains of monoclonal antibody hydridoma cells were obtained by IFA, and all were specific to MDV infected CEF through dot ELISA.
腹水经 PBS稀释后与 MDV感染的 CEF做斑点酶联免疫吸附试验 ,呈特异性显色。
2.
The Mab can also identify CEF infected by MDV through dot ELISA.
1∶ 1 0 0稀释时与 GA感染的 CEF在斑点酶联免疫吸附试验 ( dot- EL ISA)中呈阳性。
6)  ELISPOT
酶联免疫斑点试验
1.
HIV-1-specific T lymphocyte responses directed against 249 overlapping peptides spanning HIV-1 Pol gene were analyzed by an IFN-γ Elispot assay.
方法:本研究以25名HIV-1B/C重组毒株慢性期感染者为研究对象,应用酶联免疫斑点试验(Elispot)测定技术综合测定了针对覆盖HIV-1Pol基因的249条重叠多肽产生γ干扰素的特异性T淋巴细胞免疫反应。
补充资料:酶联免疫吸附试验


酶联免疫吸附试验


酶联免疫吸附试验是将可溶性抗原或抗体吸附(包被)于固相载体(目前常用微孔反应板)上,加入待检样品,所测未知抗体或抗原与包被的抗原或抗体结合,形成抗原抗体复合物,待依序加入酶结合物(酶标记抗体或酶标记抗原)和该酶的底物时,产生呈色反应。呈色程度与检样中待测抗体或抗原的量相关,可据此定性或定量。用于检测精液或宫颈黏液中的抗精子抗体,并行定量分析。方法是以精子或精子抗原提取物放入微试管,加入被测精液或宫颈黏液及抗免疫球蛋白—酶结合物,再加入酶底物观察颜色变化,以自动计算器进行定量测定。
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
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