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1)  Enzyme-Linked Immunospot
酶联免疫斑点实验
2)  enzyme-linked immunospot(ELISPOT) assay
酶联免疫斑点实验(ELISPOT)
3)  Dot-ELISA
斑点-酶联免疫吸附试验
4)  Dot-ELISA
斑点酶联免疫吸附试验
1.
Checking Salmonella in Animal Feed by Dot-ELISA;
应用斑点酶联免疫吸附试验快速检测饲料中的沙门氏菌
2.
[Objective]To detect the anti-42000 protein from egg nucleus antibody in systemic lupus erythematosus(SLE)patients with Dot Enzyme linked immunosorbent assay(Dot-ELISA),so as to explore the relationship between the anti-42000 protein from egg nucleus antibody and the correlative antibodies in SLE patients and to study it s role in the course of disease occurrence.
[目的]采用斑点酶联免疫吸附试验(Dot-ELISA)法检测系统性红斑狼疮(SLE)患者血清中抗鸡卵核42000蛋白的抗体,探讨该抗体与SLE患者相关抗体检测指标的关系,以进一步研究该抗体在SLE患者发病过程中的作用。
3.
The McAb can also identify CEF infected by MDV through dot-ELISA.
1∶ 10 0稀释时与 GA株感染的 CEF在斑点酶联免疫吸附试验 (dot- EL ISA)中呈阳性。
5)  dot ELISA
斑点酶联免疫吸附试验
1.
3 strains of monoclonal antibody hydridoma cells were obtained by IFA, and all were specific to MDV infected CEF through dot ELISA.
腹水经 PBS稀释后与 MDV感染的 CEF做斑点酶联免疫吸附试验 ,呈特异性显色。
2.
The Mab can also identify CEF infected by MDV through dot ELISA.
1∶ 1 0 0稀释时与 GA感染的 CEF在斑点酶联免疫吸附试验 ( dot- EL ISA)中呈阳性。
6)  ELISPOT
酶联免疫斑点试验
1.
HIV-1-specific T lymphocyte responses directed against 249 overlapping peptides spanning HIV-1 Pol gene were analyzed by an IFN-γ Elispot assay.
方法:本研究以25名HIV-1B/C重组毒株慢性期感染者为研究对象,应用酶联免疫斑点试验(Elispot)测定技术综合测定了针对覆盖HIV-1Pol基因的249条重叠多肽产生γ干扰素的特异性T淋巴细胞免疫反应。
补充资料:酶联免疫吸附实验


酶联免疫吸附实验
enzyme linded immunosorbent assay,ELISA

即将半抗-HBS包被于反应极凹孔底部,加入待检血清,如其中含有HBsAg则形成抗原、抗体复合物,再加入用辣根过氧化物酶或碱性磷酸酶标记的半抗-HBS,形成抗原、抗体及酶标记抗体复合物,当加入底物盐酸苯二胺后,如果HBsAg阳性则显示橙黄色,可用目测或光电比色判定结果。此法敏感度和固相放射免疫法相同,也可用以检测抗-HBS。临床已广泛用于许多疾病的病原学诊断和一些细胞因子的测定。
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