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1)  BrdU incorporation method
BrdU掺入法
1.
Methods With BrdU incorporation method,the effect of bFGF on the proliferating rate of olfactory epithelium ensheathing cells was observed in the research.
 方法 应用差速贴壁和酶消化法对原代培养的嗅黏膜OECs进行纯化 ,用BrdU掺入法观察bFGF对嗅黏膜OECs增殖率的影响。
2)  Brdu method
Brdu法
1.
Methods:UC-MSC were separated from umbilical cord,the purity of MSC was analyzed by flow cytometry; The proliferation of allogeneic T lymphocytes was measured by Brdu method;The impact on the T cells subset and cytokine secretion were measured.
方法:从脐带中分离和培养间充质干细胞,流式细胞术检测其表面标志以鉴定纯度;用Brdu法测定细胞增殖率,观察UC-MSCs对异体T淋巴细胞增殖的影响;流式细胞仪检测共培养体系中CD4+、CD8+水平检测及UC-MSCs对T淋巴细胞转化的影响;且用ELISA法检测共培养上清细胞因子IL-4、IFN-γ的表达水平。
3)  Brdu-Feulgen method
Brdu-Feulgen法
4)  H3-dTTP
H3-掺入法
5)  way of addition
掺入方法
6)  Brdu-anti-Brdu immocychemical method
Brdu-Anti-Brdu免疫细胞化学法
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