1) [3H]leucine intake method
[3H]标记亮氨酸掺入法
1.
Methods Using cultured neonatal rat cardiomyocytes treated with different factors as a model,surface area of cardiomyocytes was measured by computer photograph analysis software;the protein synthesis rate was assayed with leucine intake method;the proto-oncogene c-fos protein expression was assessed with immunocytochemistry technique;the markers of cardiom.
方法以培养的乳鼠心肌细胞为模型并分组给药,用计算机图像分析软件测量心肌细胞表面积,[3H]标记亮氨酸掺入法测定心肌细胞蛋白质合成速率,免疫细胞化学方法检测心肌细胞原癌基因c-fos蛋白表达,半定量RT-PCR检测心肌细胞肥大特征性胚胎型基因β-肌球蛋白重链(β-MHC)、α-骨骼肌肌动蛋白(α-skA)和心房肽(ANP)的mRNA表达。
2) 3H-proline incorporation
3H-脯氨酸掺入法
1.
By culturing 1,12,24 and 48 h after the irradiation,the synthesis of collagen was measured by 3H-proline incorporation determination.
于照射后不同时间段,采用3H-脯氨酸掺入法分别两组细胞胶原合成情况。
3) Incorporation of labelled glutamine
标记谷氨酸掺入
4) 3 H leucine
3H-亮氨酸
1.
By incorporation 3 H leucine,using liquid scintillation counter,we now know there was protective protein synthesis du.
引入 3H-亮氨酸 ,利用液闪技术 ,已直接从因果关系上证实保护性蛋白在预适应保护中的作用。
5) labeled leucine
标记亮氨酸
6) ~3H-Leucine corporation
氚-亮氨酸掺入实
补充资料：崭亮
1.很亮;鲜亮。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
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