RNAi is the process that double-stranded RNA induces the homology-dependent degradation of cognate mRNA mediated by 21-23 nucleotide short interfering RNA ( siRNA ) .

Construction and identification of a vector expressing short hairpin RNA targeting annexinⅡgene;

The effect of short hairpin RNA on hTERT expression;

Objective To clone shRNA (short hairpin RNA) recombinant plasmid targeting on COX-2 gene and analyze the nucleic acid sequence of the recombinant plasmid.

Inhibition of ICAM-1 gene expression in liver cancer cells by shRNA;

Objectives To investigate nano-carrier G9 PAMAM mediated plasmid containing CTGF-shRNA (pCTGF-shRNA) transfection on connective tissue growth factor (CTGF) expression in mouse peritoneal mesothelial cells (MPMCs).

The annealed oligonucleotides were inserted into the linearized pSIREN-RetroQ vector to construct the recombinant shRNA plasmid(pSIREN-VEGF-C) and then transformed into E.

The dsRNA introduced or generated in cells is subjected to digestion with a dsRNA-specific endonuclease, Dicer, into 21-25 nucleotide (nt) RNA duplexes, and the resultant duplexes, referred to as short interfering RNAs (siRNAs) duplexes, function as essential sequence specific mediators of RNAi in the RNA-induced silencing complexes (RISC).

Objective: To transfect a recombinant short hairpin RNA(shRNA)expression vector targeting Livin gene isoform(BIRC71,BIRC72)into the cervical cancer cell line(Hela cell),in an attempt to observe RNAi-mediated silen- cing on Livin gene and the induction of Hela apoptosis.