1) REF-SSCP
限制性酶切-单链构象多态性
1.
Methods:The SRY gene segment in the genomic DNA was amplified by polymerase-chain reaction(PCR); the PCR products were screened mutations using restriction endonuclease fingerprinting-single strand conformation polymorphism(REF-SSCP); mutations were confirmed by DNA sequencing.
方法应用PCR扩增SRY基因;PCR产物通过限制性酶切-单链构象多态性方法检测未知突变;基因测序以进一步明确突变。
2) restriction endonucleases fingerprinting single strand conformation polymorphism
限制酶切指纹-单链构象多态性
3) Single strand conformational polymaphism
聚合酶链反应-限制性片段长度多态性、单链构象多态性分析
4) Single strand conformation polymorphism
单链构象多态性
1.
Detection of vector gene mutation by single strand conformation polymorphism with capillary electrophoresis;
毛细管电泳单链构象多态性分析检测质粒中基因突变
2.
Detection of Leber hereditary optic neuropathy using single strand conformation polymorphism analysis;
单链构象多态性分析检测Leber遗传性视神经病变
3.
Mutations of exon 1 and exon 7 were analyzed by single strand conformation polymorphism analysis of polymerase chain reaction products(PCR-SSCP).
方法分别应用免疫组化和聚合酶链反应-单链构象多态性分析(PCR-SSCP)技术对62例膀胱癌及18例膀胱良性病变组织(对照组)中PTEN的表达及其第1和第7个外显子的突变情况进行检测。
5) single-strand conformation polymorphism
单链构象多态性
1.
The Effect of Different Gel Preparation on Detecting Mutation of AKT2 Gene by single-strand conformation polymorphism;
两种配制胶对单链构象多态性筛查AKT2基因突变的影响
2.
Application of restriction fragment length polymorphism and single-strand conformation polymorphism technique in differentiation of E.c oli serotype;
运用限制性长度多态性和单链构象多态性技术鉴别大肠杆菌血清型
3.
The leukocyte DNA was extracted and amplified by the polymerase chain reaction (PCR),and DNA sequence was analyzed by single-strand conformation polymorphism (sscp) and direct determine method.
通过外周血人基因组DNA提取,采用聚合酶链式反应(PCR)扩增,非同位素标记单链构象多态性(SSCP)分析,硝酸银染色法及直接测序法。
补充资料：限制性片段长度多态性
分子式：
CAS号：
性质：又称限制片长多态性。每一种基因型的DNA对某些限制性内切酶有固定的酶切位点，经酶作用后，在凝胶电泳图谱上产生固定的片段图谱。如基因型发生变化，则酶切位点或数目可能有差异，则产生的电泳图谱会有差异。这方法常用于遗传基因型的检查或病理检查。一般来讲高等生物个体都有特定的RFLP。
CAS号：
性质：又称限制片长多态性。每一种基因型的DNA对某些限制性内切酶有固定的酶切位点，经酶作用后，在凝胶电泳图谱上产生固定的片段图谱。如基因型发生变化，则酶切位点或数目可能有差异，则产生的电泳图谱会有差异。这方法常用于遗传基因型的检查或病理检查。一般来讲高等生物个体都有特定的RFLP。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条