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1)  immuno-polymerase chain reaction assay
Immuno-PCR
2)  polymerase chain reaction(PCR)
PCR
1.
Using the primers,7 LABs were identified by polymerase chain reaction(PCR) amplification of the 16S rDNA.
并用该引物对从啤酒厂分离到的7种乳酸菌进行了检测,PCR结果表明该引物能够准确检测到啤酒中常见的乳酸菌。
2.
Cerebrospinal fluid specimens were boiled or extracted DNA by the extraction kit and crgA gene was detected by Polymerase Chain Reaction(PCR).
[方法]以煮沸法裂解细菌获取PCR反应模板,或以基因组DNA纯化试剂盒提取DNA后,用PCR法扩增脑膜炎奈瑟菌crgA基因;对PCR产物进行DNA测序。
3.
A 16S rRNA-based polymerase chain reaction(PCR) method was used in th.
方法:分别提取26例根管预备后约诊期间炎症急性发作患牙根管样本和23例慢性根尖周炎患牙根管样本,提取样本细菌DNA,利用细菌16S rRNA引物通过PCR扩增方法鉴定细菌。
3)  Polymerase chain reaction
PCR
1.
Construction of the standards for detecting human hepatocyte growth factor mRNA with real-time fluorescence quantitative polymerase chain reaction;
人肝细胞生长因子mRNA实时荧光PCR定量标准的构建
2.
Detection of Mycoplasma gallisepticum and M. synoviae by polymerase chain reaction;
PCR检测鸡毒霉形体和鸡滑液囊霉形体的研究
4)  Polymerase Chain Reaction (PCR)
PCR
1.
Sex determination of bovine embryo using consecutive and multiplex polymerase chain reaction (PCR);
连续多重PCR牛胚胎性别鉴定
2.
Some of these techniques, such as Fluorescent in situ Hybridization (FISH), Polymerase Chain Reaction (PCR), DNA cloning and DNA sequencing, were reviewed.
综述了荧光原位杂交(FISH)、多聚酶链式反应(PCR)、DNA克隆及DNA测序等分子生物学技术,并将这些分子生物学技术应用到淡水水体底泥厌氧氨氧化菌(anammox菌)和好氧氨氧化菌的原位检测中,从底泥样品中鉴别出这两种细菌,其中好氧氨氧化菌属于亚硝酸单胞菌属,厌氧氨氧化菌属于anammox菌的Brocadia分支,为进一步研究淡水环境中氮的微生物循环过程提供了一定的依据。
3.
Objective To develop a polymerase chain reaction (PCR) for specifically detecting all serogroups of leptospira interrogans epidemic in China.
目的 建立一种能特异检测我国致病性钩端螺旋体所有血清型的PCR方法。
5)  PCR-RFLP and PCR
PCR-RFLP及PCR
6)  polymerase chain reaction(PCR) apparatus
PCR机
补充资料:countercurrent immuno electrophoresis
分子式:
CAS号:

性质:简称对流电泳,免疫电渗电泳。是一种将双向扩散和电泳技术相结合的免疫分析方法,制备琼脂板方法与免疫扩散电泳相同,但在板上挖两个小孔,距离约4mm左右。一孔中加入待测蛋白质样品,另孔中加入该蛋白质的抗血清,进行电泳,此时被测蛋白质(作为抗原)向阳极泳动,而其相应的抗体由于等电点很高及琼脂的电渗作用,向阴极泳动。结果在两孔之间的某处二者相遇并在适当的浓度下形成沉淀线,对流电泳常用来检查病人血清中的特殊蛋白质抗原,如肿瘤抗原、澳大利亚抗原等。本方法限制了抗原、抗体自由扩散的倾向。使两者相对浓缩集中,提高了分析的敏感性,缩短了分析时间(30~90min),但缺乏分辨力。

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