1) Yeast surface display
酵母表面呈现
1.
Methods The human CD55 open reading frame was cloned with PCR from CD55 pBluescript M13 (Amp+)plasmid,then subcloned into the yeast surface displaying vector pYD1.
方法 PCR从CD5 5 pBluescriptM13质粒扩增出全长的CD5 5cDNA ,酶切后克隆到酵母表面呈现载体pYD1,构建CD5 5 pYD1重组质粒后 ,转化酵母细胞。
3) Phage display
噬菌体表面呈现
1.
Construction and preliminary selection and identification of phage display library of anti-human ovarian cancer single chain Fv antibody;
抗人卵巢癌单链抗体噬菌体表面呈现文库的构建、初步筛选及鉴定
2.
Phage display technology has been applied in many biotechnological fields.
噬菌体表面呈现技术已被广泛地应用于生物技术的许多领域。
3.
Human monoclonal antibody (MAb) Fab fragments to the rabies virus have been developed by using phage display technology for the first time.
运用噬菌体表面呈现 (phagedisplay)技术获得了人源抗狂犬病毒糖蛋白基因工程单克隆抗体Fab段基因及其表达。
5) Yeast surface display
酵母表面展示
1.
The PCR products were cloned into yeast surface display vector pYD1 after digestion with enzymes, and the recombinant vector DAF-pYD1 was thus constructed.
以hDAFcDNA为模板 ,用随机引物分别通过大引物PCR和交叠PCR方法引入突变 ,酶切后克隆到酵母表面展示载体pYD1 ,构建DAF pYD1重组质粒 ,转化酵母细胞 ,PCR鉴定随机性 ,流式细胞仪分析DAF的表达。
6) Phage display
噬菌体表面呈现技术
补充资料：呈现
显露；表现：湖光山色呈现在眼前｜呈现出缤纷的色彩。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条