1)  Reverse transcription polymerase chain reaction
逆转录-聚合酶 链反应
2)  reverse transcription polymerase chain reaction
逆转录聚合酶链反应
1.
[Methods] The level of human Fascin1 gene in endometrial carcinoma tissues(study group,n=30) and normal endometrial tissues(control group,n=10) was investigated by reverse transcription polymerase chain reaction.
方法选取30例新鲜子宫内膜癌组织标本(实验组)及10例正常子宫内膜组织标本(对照组),应用逆转录聚合酶链反应(RT-PCR)研究人类Fascin1基因的表达情况,并分析其与组织学类型、临床分期、病理分级、雌孕激素受体状态的相关性。
2.
After 1,4,7,10,14 days of exposure,the rats were killed,whole lung RNA were isolated and ATPase6,8 mRNA were detected by reverse transcription polymerase chain reaction(RT-PCR).
二组分别于实验后1、4、7、10和14 d提取其肺组织RNA,采用半定量逆转录聚合酶链反应(RT-PCR)测定ATPase6、8 mRNA表达。
3.
Methods COX-2 mRNA expression was determined by semi-quantitative reverse transcription polymerase chain reaction(RT-PCR),and cell proliferation analyzed by cell counting kit-8(CCK-8) assay after exposure to dicl.
方法采用半定量逆转录聚合酶链反应(RT PCR)检测各种细胞系COX 2 mRNA的表达水平,采用cell counting kit 8(CCK 8)细胞计数法测定药物作用72 h后的细胞增殖活性,绘制生长曲线。
3)  Reverse transcription-polymerase chain reaction
逆转录-聚合酶链反应
1.
Expression of the TFPI-2 gene was determined by Reverse transcription-polymerase chain reaction(RT-PCR) and Western blotting.
方法:将重组质粒pEGFP-C1-TFPI-2通过脂质体介导转染胰腺癌细胞系Panc-1细胞,G418筛选获得阳性细胞克隆后,用逆转录-聚合酶链反应(RT-PCR)和免疫印迹(Western blot)技术分别检测转染细胞中TFPI-2 mRNA及相应蛋白的表达,同时测定转染细胞的生长曲线和凋亡情况。
2.
Methods:Reverse transcription-polymerase chain reaction(RT-PCR) was used to semiquantite the expressions of GRα and β mRNA in PBMC of 18 cases of PM/DM patients sensitive to GC,10 cases of PM/DM patients resistant to GC and 20 cases of health blood donors.
方法采用逆转录-聚合酶链反应(RT-PCR)方法对18例GC治疗敏感、10例GC治疗抵抗的PM/DM患者PBMC中GRα、β mRNA的表达水平进行了检测,同时采用放射免疫分析法测定血清中的皮质醇含量,并与20例正常对照进行比较。
3.
Methods Using reverse transcription-polymerase chain reaction(RT-PCR),the PIM3 mRNA expression in normal murine ocular tissues was defected.
方法应用逆转录-聚合酶链反应及免疫组化检测PIM3在小鼠眼部组织中的表达。
4)  Reverse transcriptase polymerase chain reaction
逆转录聚合酶链反应
1.
Methods The p24 gene fragment of BDV in PBMCs or CSFs in these patients infected in intracalvarium were examined by nested reverse transcriptase polymerase chain reaction(RT-PCR) and .
方法用荧光定量巢式逆转录聚合酶链反应(FQ-nRT-PCR)方法检测不明原因颅内感染患者及对照者PBMCs或CSFMCs中BDV p24基因片段,同时用β-肌动蛋白(-βactin)作为内参照,并总结阳性患者的临床特征。
2.
Methods: The p24 fragment of BDV in peripheral blood mononuclear cells(PBMC) in 52 patients with viral encephalitis and 30 healthy donors were examined by nested reverse transcriptase polymerase chain reaction(RT-PCR) and fluorescence quantitative(FQ) PCR.
方法:用荧光定量巢式逆转录聚合酶链反应(FQ-n RT-PCR)方法检测病毒性脑炎患者及正常体检者PB-MCs中BDVp24基因片段,同时用β-肌动蛋白(β-actin)作为内参照,总结出临床特征。
3.
The two consecutive fragments of human U5·116 ku were amplified by reverse transcriptase polymerase chain reaction (RT-PCR).
方法:从HeLa细胞中提取总体RNA,一步法合成单链cDNA,利用逆转录聚合酶链反应(RT-PCR)法,扩增出人类U5·116 ku基因两段连续的序列,首先分别克隆至pTZ57R/T载体,两段序列连接成全长后再定向克隆至真核表达载体pcDNA3。
5)  RT-PCR
逆转录-聚合酶链反应
1.
Methods:Reverse transcription polymerase chain reaction(RT-PCR) was used to detect the expression of RASSF1A mRNA in 29 cases of human transitional cell carcinoma of bladder and 5 cases of normal bladder tissues.
方法:采用逆转录-聚合酶链反应(RT-PCR)检测29例膀胱移行细胞癌组织及5例正常膀胱组织中RASSF1A mRNA的表达水平。
2.
Results:RT-PCR and the sequence analysis of its product suggested that these 58 strains of measles viruses should belong to the H1 genotype of the eighth genetic group of wild measles virus.
结果:该58株病毒经逆转录-聚合酶链反应和其产物的基因序列分析显示,这58株病毒属于麻疹野病毒第8基因组H1基因型。
3.
Methords: The expression of PCA-1 mRNA was detected by RT-PCR in the samples from 45 cases of PCa with various clinico-pathologic characteristics, 30 cases of high-grade prostatic intraepithelial neoplasia (HG-PIN), 43 cases of BPH and 39 cases of other carcinorma tissues.
方法:采用逆转录-聚合酶链反应(RT-PCR)技术,检测45例PCa组织、30例前列腺高分级上皮样内瘤样病变组织(HG-PIN)、43例BPH组织和39例其他肿瘤组织标本中PCA-1 mRNA的表达。
6)  RT-PCR
逆转录聚合酶链反应
1.
METHODS: The Balb/c mice were treated with saline, BCG-PSN and lipopolysaccharide(LPS) respectively, and mIFNγ cDNA was amplified by RT-PCR.
方法:分别采用生理盐水、卡介菌多糖核酸和脂多糖处理Balb/c小鼠,逆转录聚合酶链反应(RT-PCR)技术扩增编码小鼠干扰素γ(mIFNγ)cDNA,通过RT-PCR产物来评价卡介菌多糖核酸诱导mIFNγ表达的能力,并设β-actin为对照。
2.
RT-PCR was performed for the hTERT mRNA in urine exfoliated cells.
方法采用逆转录聚合酶链反应(reverse transcriptase PCR,RT-PCR)检测42例膀胱癌、38例膀胱良性疾病患者和12例健康体检者晨尿中脱落细胞hTERT mRNA的表达,并与尿脱落细胞学检查结果比较;随访观察其中37例膀胱癌术后尿液hTERT mRNA变化与复发的关系。
3.
Methods Two siRNAs against the VEGF gene were designed and transfected into T24 cells respectively,VEGF gene expression was measured using RT-PCR.
方法体外构建两个针对VEGF基因的siVEGF的重组质粒,转染T24细胞;应用逆转录聚合酶链反应(RT-PCR)测定VEGF基因的表达,并用MTT法和流式细胞仪检测siVEGF对细胞的增殖抑制率和凋亡的影响。
参考词条
补充资料：聚合酶链反应


聚合酶链反应
polymerase chain reaction,PCR

是用特异性寡核苷酸引物在DNA聚合酶的作用下对靶DNA序列进行大量扩增的分子生物学方法。PCR技术中以在短时间内把极其微量的特定DNA(或RNA）片断大量增扩，达到用常规方法就可以检测的水平。临床用来确定一些疾病的病原体，因其假阳性率较高，故应谨慎使用。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。