1) line immuno assay
线性印迹法
1.
Methods:The anti-SmD1 antibody in 137 cases with SLE and 75 cases without SLE were measured by line immuno assay(LIA).
方法:将137例确诊为SLE患者和75例非SLE患者先采用线性印迹法定性检测SmD1。
2) The Antinuclear antibody spectral line blot
抗核抗体谱线性印迹法
1.
Methods The Antinuclear antibody spectral line blot was used to measure the AnuA in serum of 59 SLE patients,20 patients with other rheumatic diseases(including primary SjSgren syndrome,systemic sclerosis,rheumatoid arthritis.
AnuA、Sm、抗组蛋白抗体,核糖体P蛋白抗体则采用德国欧蒙公司抗核抗体谱线性印迹法检测。
3) Western blotting
Western印迹法
1.
Using western blotting and MTT chemosensi-tive testing, the relationship between the expression of NFkB family proteins, P53 and MDM2 in ovarian cancer cell lines and chemotherapy resistance of ovarian cell was ob.
培养13个卵巢癌细胞系,提取癌细胞蛋白,采用Western印迹法观察NFkB家族蛋白、P53和MDM2的表达,并用四氮唑盐法药敏试验观察与卵巢癌细胞化疗耐药性关系。
2.
Using the mouse antibody to Hsp70of human,pig,dog,etc,Hsp70was detected from seven randomly sele cted yeast strains by Western blotting.
随机抽取其中7株新生隐球菌,采用鼠抗人、猪、狗等的Hsp70抗体,通过Western印迹法来检测其Hsp70,根据实验信号强弱定性判断在不同刺激条件下的Hsp70产量。
3.
Using Western blotting and MTT chemosensitive testing, the relationship between the expression of NFκB family proteins in ovarian cancer cell lines with chemotherapy resistance of ovarian cell was observed.
方法 :培养 13株卵巢癌细胞系 ,对其进行胞质、胞核蛋白质提取 ,Western印迹法观察NFκB家族蛋白及其抑制物的表达 ,并用MTT检测药敏、观察NFκB家族蛋白与卵巢癌细胞化疗耐药性关系。
4) Blotting
[英][blɔt] [美][blɑt]
印迹法
1.
After serum starving for 24 hours, the cells were exposed to AGEs (50 mg/L、100 mg/L) for 12, 24 and 48 hours respectively and western blotting was used to detect the expression of p57, p27, p21 and hhLIM.
应用Western蛋白印迹法检测不同浓度AGEs(5 0mg L、10 0mg L)对心肌细胞刺激 12、2 4、4 8h后细胞中p5 7、p2 7、p2 1及hhLIM蛋白表达情况。
2.
, we detected 6 clinically isolatedstrains and 1 CZYD2 standard strain of Cryptococcus neoformans by Western blotting.
方法：采用鼠抗人、猪、狗等的Hsp70抗体通过Western印迹法检测6株临床分离株及1株CZYD2标准株新生隐球菌的 Hsp70，根据实验信号强弱定性判断在不同刺激条件下的 Hsp70产量。
3.
After selection by G418, resistant clones were screened by RT PCR and Western blotting test.
挑取单细胞克隆 ,RT PCR及蛋白质印迹法鉴定NS1基因的稳定表达。
5) Western blot
Western印迹法
1.
Methods Western blot and immunohistochemistry were performed to detect the expression and phosphorylation status of GluR1.
方法采用Western印迹法、免疫组织化学法检测纹状体AMPA受体亚单位GluR1表达及磷酸化状态。
补充资料：“诺森”印迹法
分子式：
CAS号：
性质：又称RNA印迹（法）。与DNA斑迹法过程类似的技术。用于从凝胶中转移出RNA片段转移至硝酸纤维素膜，尼龙膜等，并用探针进行杂交（称“北方”杂交）以显示目的区段的位置。显示RNA的大小及丰度。
CAS号：
性质：又称RNA印迹（法）。与DNA斑迹法过程类似的技术。用于从凝胶中转移出RNA片段转移至硝酸纤维素膜，尼龙膜等，并用探针进行杂交（称“北方”杂交）以显示目的区段的位置。显示RNA的大小及丰度。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条