Taking the seeds of disease resistant pumpkin germplasm 482-2 as material,the pumpkin regeneration in vitro was studied.

Brassica campestris was hard to regeneration in vitro, while gene engineer and otherhigh .

[Objective] The research aimed to study the conditions that affected the establishment of in vitro regeneration system in Momordica charontia L.

The main contents of the experiment were as follows: establishment of the high-effeciency in vitro regeneration system of peach and pear; In vitro conservations included restricting growth methods built on plant tissue culture and changes of trace elements on STN(shoot-tip necrosis) and normal seedlings in the in vitro culture of peach embryo seedings and so on, wh.

for establishing its in vitro regeneration system.

Factors Affected Plant Regeneration Frequency of in Vitro Cultured Radish (Raphanus stavius L.);

On the purpose of increasing the inducible frequency of carrot somatic embryogesis,giving reference to high efficiency of carrot regeneration and transformation,the author reviewed the main factors influcing the establishmen.

This paper comprehensive describes the basic research from the actual requirement, which includes seed germination conditions, plant regeneration, callus induction and subculture, the physiological and biochemical characters on the cell suspension culture and the anatomy structure of vegetable organs.

Advances in biotechnological research on Narcissus,including in vitro plant regeneration,gene cloning,genetic transformation as well as their applications to micro propagation,virus free,transgenic improvement,germplasm and manufacture of secondary metabolite are reviewed,and the existing problems and intending prospects are also indicated.