1)  ISH
原位杂交
1.
A Convenient Non-radio ISH Method for Detection of Pancreatic Elastase Ⅱ mRNA;
一种简便的非放射性原位杂交法检测胰腺Ⅱ型弹性蛋白酶mRNA
2.
Using ISH to Study the Expression and Distribution of FcRn in the Small Intestine of the Piglet;
利用原位杂交技术研究仔猪小肠FcRn表达及分布规律
3.
Methods :ISH were used to determine the effect of Ganoderma appanatum polysaccharides GF on N-ras 、C-myc mRNA in mouse HepA tumor.
方法:应用地高辛标记探针原位杂交技术,检测树舌多糖GF对HepA瘤细胞中癌基因N-ras、C-myc mRNA丰度的影响。
2)  ISH
ISH
1.
Then, application of genomic in situ hybridization(GISH) in plant cytogenetics research as well as fluorescence in situ hybridization(FISH) in plant physical mapping and chromosome identification were detailed reviewed.
DNA分子原位杂交(in situ hybridization,ISH)是植物分子细胞遗传学研究的重要工具。
2.
Objective: To explore the value of HER-2/neu and its gene product (cerbB-2)、HPV and P16 in cervical lesions by using CISHISH、and IHC、and to detect their differences and expression、correlation of the four indicators.
目的:研究显色原位杂交(CISH)、原位核酸杂交(ISH)及免疫组化(IHC)在宫颈病变中HER-2/neu基因及其蛋白产物CerbB-2、HPV、P16检测的应用价值,以及HER-2/neu、CerbB-2、HPV、P16在宫颈变化中的表达和相互关系。
3.
In this article we observed the expression of MK which expressed in oral malignant tumor at genic and protein levels,using RT-PCR,Western-blotting,ICH and ISH,in order to explore the clinical value of it during the treatment of disease.
本文利用临床病理标本(癌、转移淋巴结、良性肿瘤、正常口腔组织),采用RT-PCR、WESTERN-BLOTTING、免疫组织化学(ICH)、原位杂交(ISH)检测方法来观察MK 在口腔恶性肿瘤中的基因和蛋白水平的表达情况,从而探讨其在疾病治疗过程中的临床应用价值。
3)  ISH
老年纯收缩期高血压病(ISH)
4)  ISH
纯收缩期高血压
1.
Influence of Nursing Intervention on ISH Patients in Community;
护理干预对社区纯收缩期高血压病人的影响
5)  ISH
EB病毒编码小分子RNA
6)  elderly ISH
老年纯收缩期高血压
参考词条
补充资料:原位杂交
分子式:
CAS号:

性质:(1)一种克隆筛选方法。将DNA克隆涂布或点布在固体培养基表面,形成菌管或噬菌斑后,将其转移至硝酸纤维素滤膜或尼龙膜上,与同位素(或非同位素)标记的DNA(或RNA)探针(probe)杂交,以探知含有与探针同顺序列的克隆的存在。(2)DNA片段在染色体上定位的技术。应用以同位素或荧光染料标记的DNA探针与染色体DNA杂交。在光学显微镜(或荧光显微镜)下可以看到DNA探针与相互补DNA序列结合的位置。采用不同颜色的荧光标记,可以同时测定几个DNA探针的染色体定位及它们之间的序列关系。

说明:补充资料仅用于学习参考,请勿用于其它任何用途。