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1)  in situ hybridization(ISH)
原位杂交染色法
2)  in situ hybridization
原位杂交染色
1.
Methods The assay of ER mRNA was detected by in situ hybridization technique and the expression of PS2 was completed by immunohistochemical method of avidin biotin complex in the 10% neutral formalin fixed and routinely paraffin embedded sections of specimen with pancreatic carcinoma.
方法 :35例胰腺癌手术切除标本经 1 0 %中性福尔马林固定后常规制作石蜡包埋切片 ,ERmRNA染色为原位杂交染色法 ,PS2染色为常规ABC免疫组化法。
2.
Methods In situ hybridization was used determine the expression in routinely paraffin embedded sections.
方法　石蜡包块切片组织原位杂交染色法。
3.
Collected their cerebrum and cerebellum, fixed and stained with in situ hybridization .
取材大脑、小脑于 95 %乙醇中固定 ,经原位杂交染色 ,结果 :着染部位主要在大脑皮层锥体细胞及皮质下各核团的较大神经元的胞浆内 ,呈现蓝紫色连成片或单独数个较大颗粒 ,小脑蒲肯野细胞胞浆内也较多见 ;此外小胶质细胞胞浆内也发现很多呈阳性。
3)  chromosome in situ hybridization
染色体原位杂交
1.
The chromosome in situ hybridization is an important part in modern biotechnology.
染色体原位杂交技术是现代生物技术的重要组成部分。
2.
The technique of chromosome in situ hybridization (CISH) has been extensively applied to genetics and breeding of higher plants.
染色体原位杂交技术近年发展较快,已广泛应用于植物遗传育种实践。
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4)  in situ chromosome hybridization
原位染色体杂交
5)  45S rDNA FISH
染色体荧光原位杂交
1.
In this study,hybrid plants from the crosses between \'Royal Lace\' × \'High Class\' were analyzed with Giemsa C-banding and 45S rDNA FISH.
利用Giemsa C-带和染色体荧光原位杂交(45S rDNA FISH)的方法对‘Royal Lace’בHigh Class’的杂种后代分别进行了鉴定。
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6)  in situ hybridization
原位杂交法
1.
Rapid Identification of Common Bacteria and Fungus in Blood Culture by Fluorescent In Situ Hybridization;
荧光原位杂交法对血培养常见菌的快速鉴定
2.
Methods The expression of MMP9 and EBV-encoded RNA (EBER) were detected by immunohistochemistry and in situ hybridization in 59 cases of mucosal natural killer/T cell and mature T cell lymphomas.
方法用免疫组化SP法和原位杂交法检测59例黏膜NK/T以及成熟T细胞淋巴瘤MMP9和EBER表达情况,统计MMP9表达与EBV感染的关系。
3.
Methods In situ hybridization(ISH) technique was used to detect HPV-DNA (HPV 6,11,16,18,30,31,33,35,45,51,52) in condyloma acuminata tissues by HPV broad-spectrum probes.
方法　应用原位杂交法 ,通过广谱生物素标记的HPV DNA探针 ,对尖锐湿疣患者疣体HPV 6,11,16,18,3 0 ,3 1,3 3 ,3 5 ,45 ,5 1,5 2型DNA进行检测。
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补充资料：荧光原位杂交


荧光原位杂交


是以荧光素标记取代核素标记而形成的一种新的原位杂交方法。利用已知碱基序列的非核素标记的核酸探针，依据碱基配对原理，通过免疫细胞化学检测体系，在组织切片、细胞间期核或染色体等标本上，进行DNA定性、定位及定量分析。本法快速、安全、灵敏度高，特异性强。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
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