1) Repetitive element-based polymerase chain reaction
重复序列引物聚合酶链反应
1.
Repetitive element-based polymerase chain reaction to track the nosocomial infec- tions caused by Staphylococcus aureus;
重复序列引物聚合酶链反应追踪金黄色葡萄球菌所致医院感染
3) PCR-SSP
聚合酶链反应-序列特异性引物
1.
Methods A total of 192 RhD positive samples,and 166 RhD negative samples were tested by PCR-SSP for exons of RHC/E and D genes.
方法用聚合酶链反应-序列特异性引物(PCR-SSP)扩增技术,扩增Rh血型C/E基因,D基因的外显子,检测192例RhD阳性个体,166例RhD阴性个体的血样品。
2.
Methods:HLA-DRB alleles of 82 vitiligo patients and 80 psoriasis vulgaris patients were detected by polymerase chain reaction sequence-specific primer(PCR-SSP),under the comparison with 86 healthy persons.
方法:采用聚合酶链反应-序列特异性引物(PCR-SSP)分型技术,对82例汉族白癜风患者和80例寻常型银屑病患者HLA-DRB1等位基因进行检测,与86名汉族健康人群进行对照。
3.
Methods A total of 88 umbilical cord blood samples,including those randomly selected from Tianjin stem cell bank and already matched for transplantation,were performed polymerase chain reaction sequence specific primers(PCR-SSP) and sequencing based type (SBT) to check their type results and sequencing based type.
方法采用聚合酶链反应-序列特异性引物扩增(PCR-SSP)方法、直接测序分型穴SBT雪方法,随机抽查天津市脐带血造血干细胞库脐带血及查询已选中做脐带血移植的样本共88例SSOP方法的结果进行HLA基因分型复核。
4) polymerase chain reaction-sequence specific primer
聚合酶链反应-序列特异性引物
1.
Methods HLA-DR15 was detected by the technique of polymerase chain reaction-sequence specific primers(PCR-SSP) and compared between peripheral blood of MDS group(n=59)and control group(n=104).
方法采用聚合酶链反应-序列特异性引物(PCR-SSP)检测MDS患者(n=59)HLA-DR15基因的表达,并与健康对照组(n=104)进行比较。
2.
Methods Polymerase chain reaction-sequence specific primer (PCR-SSP) was used to analyze the distribution of HLA-A alleles among 106 patients with systemic lupus erythematosus and 122 healthy persons.
方法应用聚合酶链反应-序列特异性引物(PCR-SSP)的方法,对106例SLE患者和122例健康人进行HLA-A等位基因的检测。
5) PCR-SSP
聚合酶链反应-序列特异引物
1.
Methods: The HLA-DRB1 alleles were detected by a PCR-SSP technique in 34 patients with generalized vitiligo and 262 normal controls in northern China.
方法:采用聚合酶链反应-序列特异引物(PCR-SSP)技术检测34例北方汉族泛发型白癜风患者的HLA-DRB1等位基因。
2.
Methods: With high-resolution polymerase chain reaction amplification with sequence specific primers(PCR-SSP),we detected the distribution frequencies of HLA-DRB1*07 allele among 60 cases with pv and 80 healthy controls and compared.
方法采用聚合酶链反应-序列特异引物(PCR-SSP)法,检测60例寻常型银屑病患者及80名健康对照者的等位基因频率,并相互比较。
3.
Methods Using method of high-resolution polymerase chain reaction amplification with sequence specific primers (PCR-SSP),we detected the distribution frequencies of HLA-DRB1*0701 allele among 42 patients with psoriasis vulgaris,28 patients with psoriasis pustulosa and 50 healthy patients as the control,and they were compared.
方法采用聚合酶链反应-序列特异引物(PCR-SSP)法检测42例寻常性银屑病、28例脓疱性银屑病和50例健康对照者HLA-DRB1*0701等位基因频率,并相互比较。
6) Polymerase chain reaction-sequence specific primers
聚合酶链反应/序列特异性引物
补充资料：聚合酶链反应
聚合酶链反应
polymerase chain reaction,PCR
是用特异性寡核苷酸引物在DNA聚合酶的作用下对靶DNA序列进行大量扩增的分子生物学方法。PCR技术中以在短时间内把极其微量的特定DNA(或RNA）片断大量增扩，达到用常规方法就可以检测的水平。临床用来确定一些疾病的病原体，因其假阳性率较高，故应谨慎使用。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条