1) Electroporation
电穿孔转化
1.
After electroporation of the plasmid into Lactobacillus casei CECT5276, PCR using the genomic DNA of the recombinant lactobacillus as template was performed to confirm whether t.
方法 :用 PCR从短小乳酸杆菌染色体中扩增得到 S-层蛋白的信号肽序列 ,将其与 h CGβ基因连接后克隆到乳酸杆菌整合型表达载体 p Ilac的 lac启动子下游 ,得到质粒 p Ilac h CGβ,电穿孔转化干酪氏乳酸杆菌 Lb。
2.
Conjugation and electroporation were used to genetically improve these strains.
利用接合转移和电穿孔转化等方法对这些菌株进行了遗传改良研究。
2) electroporation
电穿孔转染
1.
Objective To optimize the electroporation parameters in mouse primary lymphocyte cultured in suspension with adenosine deaminase acting on RNA 1(ADAR1) as a reporter gene.
目的以RNA依赖性腺嘌呤脱氨酶(ADAR1)为靶基因优化悬浮培养的小鼠原代淋巴细胞的电穿孔转染条件。
2.
It turns out that 250mg/L carrier DNAincreases the expression level by 3 fold,andlinearized plasmid DNA is more suitable thansupercoiled DNA to obtain stable transfectionby electroporation.
以外源红细胞生成素ｃＤＮＡ的表达产物为指标，研究了运载ＤＮＡ和重组表达质粒的构象对电穿孔转染ＣＨＯ细胞的效率的影响。
3) electroporational gene transfer
电穿孔基因转移
4) electroporation
电穿孔
1.
Characteristic analysis of electroporation apparatus for transdermal drug delivery;
经皮给药电穿孔仪的性能分析
2.
Progress of electroporation in improving the efficacy of DNA vaccine;
电穿孔增强核酸疫苗免疫反应的研究进展
3.
Effect of Electric Field Direction on the Percutaneous Penetration of Caffeine Mediated by Skin Electroporation and Iontophoresis;
电场方向对咖啡因电穿孔与离子导入经皮渗透的影响比较
5) electroporation
电穿孔法
1.
Experimental research on transformation of plasmid DNA into Escherichia coli XL1-Blue MRF′ strain mediated by electroporation.;
电穿孔法介导质粒DNA转化大肠杆菌XL1-Blue MRF′菌株的实验研究
2.
The transfection efficiency of human telomerase reverse transcriptase(hTERT) in primary culture of neonatal rat cardiomyocytes is improved by electroporation;
电穿孔法提高hTERT转染原代培养乳鼠心肌细胞效率的研究
3.
Optimization of parameters of transfection of primary cultured keloids fibroblasts in vitro by electroporation;
电穿孔法转染原代培养的瘢痕疙瘩成纤维细胞的条件优化
6) piercing by electric arc
电弧穿孔
补充资料：成都无缝钢管厂管坯在菌式穿孔机上穿孔
成都无缝钢管厂管坯在菌式穿孔机上穿孔
成都无缝钢管厂管坯在菌式穿孔机上穿孔
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条