1) rotary punch
回转穿孔钳
2) Perforated patch clamp
穿孔膜片钳
3) rotary piercing mill
回转式火力穿孔机
4) perforated-patch clamp
穿孔膜片钳技术
1.
K~+ current of porcine coronary artery SMC recorded by perforated-patch clamp;
穿孔膜片钳技术使电极与细胞胞质之间保持电学连续性,它使细胞内环境保持稳定,利于研究胞内信息传导机制,并可改善常规全细胞膜片钳时破膜所造成的封接稳定性的破坏,有望提高实验的成功率。
5) Electroporation
电穿孔转化
1.
After electroporation of the plasmid into Lactobacillus casei CECT5276, PCR using the genomic DNA of the recombinant lactobacillus as template was performed to confirm whether t.
方法 :用 PCR从短小乳酸杆菌染色体中扩增得到 S-层蛋白的信号肽序列 ,将其与 h CGβ基因连接后克隆到乳酸杆菌整合型表达载体 p Ilac的 lac启动子下游 ,得到质粒 p Ilac h CGβ,电穿孔转化干酪氏乳酸杆菌 Lb。
2.
Conjugation and electroporation were used to genetically improve these strains.
利用接合转移和电穿孔转化等方法对这些菌株进行了遗传改良研究。
6) electroporation
电穿孔转染
1.
Objective To optimize the electroporation parameters in mouse primary lymphocyte cultured in suspension with adenosine deaminase acting on RNA 1(ADAR1) as a reporter gene.
目的以RNA依赖性腺嘌呤脱氨酶(ADAR1)为靶基因优化悬浮培养的小鼠原代淋巴细胞的电穿孔转染条件。
2.
It turns out that 250mg/L carrier DNAincreases the expression level by 3 fold,andlinearized plasmid DNA is more suitable thansupercoiled DNA to obtain stable transfectionby electroporation.
以外源红细胞生成素ｃＤＮＡ的表达产物为指标，研究了运载ＤＮＡ和重组表达质粒的构象对电穿孔转染ＣＨＯ细胞的效率的影响。
补充资料：成都无缝钢管厂管坯在菌式穿孔机上穿孔
成都无缝钢管厂管坯在菌式穿孔机上穿孔
成都无缝钢管厂管坯在菌式穿孔机上穿孔
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条