RESULTS: No homozygous deletion of p16 gene exon 1, 2 and 3 was observed in any of the 33 cases and no point mutation of p16 gene exon 2 was detected in 10 specimens.

Methods: Polymerase chain reaction (PCR) and sequencing analysis were used to detect the homozygous deletion and point mutation of p16 gene in 84 cases of primary hepatocellular carcinomas and their adjacent liver tissues.

The frequency of homozygous deletion was 8.

Detection of homozygous deletion and mutation of exon 5 and exon 8 of FHIT gene in differentiated thyroid carcinoma tissue;

The homozygous deletion was detectyed by multiple PCR analysis and mutation by PCR-SSCP and DNA sequencing followed.

Aim: To investigate the frequencies of homozygous deletion of entire coding exons of fragile histidine triad (FHIT) gene in laryngeal squamous cell carcinoma (LSCC) tissue and its clinical significance.

Loss of heterozygosity of chromosome 22 in sporadic colorectal carcinoma;

Methods: Mutation in exon 3 of β-catenin gene and mutation cluster region of APC gene, loss of heterozygosity and methylation status of promoter 1A of APC gene in breast cancer and normal tissue around tumor were detected by polymerase chain reaction-single strand conformation polymorphism, microsatellite analysis and methylation specific PCR.

Objective Loss of heterozygosity (LOH) of tumor suppressor gene(TSG) is believed to play a key role in carcinogenesis of colorectal cancer (CRC).

Detection of allele-specific chromosome 3p25 by PCR -LOH in 35 cases of esophageal carcinoma;

Methods: We performed restriction fragment length polymorphism (RFLP) analysis by using the polymerase chain reaction (PCR) and primer sets of two DNA markers to examine loss of heterozygosity (LOH) from 3p in glioma samples.