Methods Insert the modified HIV-1 Gag-Pol gene downstream to the promoter P7.

Methods The Gag-Pol gene sequence was amplified from wild HIV-1,then modified and inserted into constructed VR vector.

Cloning and sequence analysis of the gag gene of endogenous jaagsiekte sheep retrovirus inner mongolia strain;

Subtype and characterization of gag gene of HIV-1 strains prevalent in some areas of China

Methods Six DNA fragments of HIV-1 GAG gene were amplified by RT-PCR from blood plasma obtained from six HIV-1 carriers in Jiujiang.

By mediation of agrobacterium tumefaciens, HIV gag-gp120 gene was transferred into cotyledons of Beijing-lettuces.

Cloning and sequencing analysis 5 partial gag gene of jaagsiekte sheep retrovirus strain Inner Mongolia;

Cloning and sequence analysis of pol gene of the Inner Mongolia strain of endogenous jaagsiekte sheep retrovirus;

Objective To investigate the epidemic of the antiretroviral resistance HIV-1 strains in antiretroviral drug-naive and drug-used AIDS patients in some Chinese counties, to evaluate the effect of AIDS patients with antiretroviral therapy in Henan province, and to probe into the variabilities of quasispecies and the phylogenetic analysis of HIV-1 pol gene tentatively.

A SYBR Green I-based real-time PCR was developed for detection of ALV by using a pair of primers that was designed to target conservative region of pol gene.