1) SELDI-TOF-MS
表面增强激光解吸电离飞行时间质谱
1.
Objective:To detect the serum proteomic patterns in lung cancer by surface enhanced laser desorption/ ionization-time of flight-mass spectrometry (SELDI-TOF-MS) protein chip array techniques,screen biomarkers of lung cancer and build diagnostic models in order to evaluate their clinical significance.
目的:探讨用表面增强激光解吸电离飞行时间质谱(SELDI-TOF-MS)技术筛查肺癌血清特异性蛋白质的临床意义。
2.
The proteins were analyzed by surface enhanced laser desorption/ionization time-of-flight mass spectra(SELDI-TOF-MS),compared with other bacteria,and the data was collected by Ciphergen Protein Chip software.
方法选取分枝杆菌标准菌株,提取细菌蛋白,干化学法测蛋白浓度,应用表面增强激光解吸电离飞行时间质谱技术(SELDI-TOF-MS)检测分枝杆菌蛋白表达,和非分枝杆菌蛋白指纹图谱比较,筛选分枝杆菌特征性蛋白。
3.
Methods The Surface enhanced laser desorption/ionization time-of-flight mass spectrometry(SELDI-TOF-MS) and CM10 ProteinChip technology were used to analyze the serum proteins spectrum in 33 cases of AFP-negative PHC, 35 cases of healthy people, 30 cases of AFP-negative CLD, 28 cases of low AFP level PHC and 34 cases of low AFP level CLD.
方法:运用表面增强激光解吸电离飞行时间质谱(SELDI-TOF-MS)技术,选择弱阳离子交换芯片(CM10),检测33例AFP阴性肝癌患者与35例健康人/30例AFP阴性慢性肝病患者、28例AFP低浓度阳性肝癌患者与34例AFP低浓度阳性慢性肝病患者的血清蛋白表达谱,并用Ciphergen ProteinChip Software 3。
2) SELDI-TOF-MS
表面增强激光解吸电离-飞行时间质谱
1.
Application of SELDI-TOF-MS technology analyzing serum protein profiling in diagnosis of thyroid cancer;
表面增强激光解吸电离-飞行时间质谱在甲状腺癌诊断及临床分期中的应用
3) SELDI-TOF-MS
表面增强激光解吸/电离飞行时间质谱仪
1.
Methods Proteomic spectra were generated by surface-enhanced laser desorption/inionation-time of flight-mass spectra (SELDI-TOF-MS) and weak cation exchange protein chip system.
方法采用弱阳离子结合芯片(WCX2)及表面增强激光解吸/电离飞行时间质谱仪(SELDI-TOF-MS)检测食管癌高发区无症状普查人群143人(其中活检组织诊断为正常63人,CAG57人,DYS23人)的血清蛋白质质谱,对原始信号的总离子强度及相对分子质量做均一化校正及噪声滤过,并对同一质荷比蛋白质质谱峰平均强度值做组间t检验。
4) surface enhanced laser desorption /ionizationtime of flight-mass spectrometry(SELDI-TOF-MS)
表面增强激光解吸/电离-飞行时间-质谱(SELDI-TOF-MS)
5) surface-enhanced laser desorption ionization time of flight mass spectrometry technology
表面增强激光解吸电离子-飞行时间质谱技术
6) surface-enhanced laser desorption/ionization-time of flight mass spectrometr
表面增强激光解吸电离飞行时间质谱(SELDI-TOF-MS)
补充资料:基质辅助激光解吸时间飞行质谱
分子式:
CAS号:
性质:从原理上讲,它属于激光解吸质谱(LDMS),它与普通LDMS的不同在于,激光不是直接打在试样上使之解吸,而是把试样悬浮在基质中,激光打在基质上,基质吸收并传递激光能量,使基质中的试样解吸出来,试样解吸并电离后进入时间飞行质谱仪中检测。MALDI-TOFMS由于使用了基质辅助,它对激光能量的限制就比较宽了,对不同的试样,改变基质,可以获得更满意的结果。目前MALDI-TOF MS是用来进行药物、生物分子的测定的较好手段。
CAS号:
性质:从原理上讲,它属于激光解吸质谱(LDMS),它与普通LDMS的不同在于,激光不是直接打在试样上使之解吸,而是把试样悬浮在基质中,激光打在基质上,基质吸收并传递激光能量,使基质中的试样解吸出来,试样解吸并电离后进入时间飞行质谱仪中检测。MALDI-TOFMS由于使用了基质辅助,它对激光能量的限制就比较宽了,对不同的试样,改变基质,可以获得更满意的结果。目前MALDI-TOF MS是用来进行药物、生物分子的测定的较好手段。
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
参考词条