1) Melting curve
熔解曲线
1.
Influence of experimental conditions on probe melting curves based on fluorescence resonance energy transfer;
实验条件对荧光共振能量转移的探针熔解曲线影响
2.
Melting curve of target DNA fragments were drawn using real-time PCR through Light Cycler Instrument integrated with specific oligonucleotide probes,and the samples were genotyped according to the peak of the melting tempera.
通过LightCycler实时PCR仪描绘扩增的目标DNA片段的熔解曲线,根据熔解温度(Tm)峰值判定待测标本的基因变异类型。
2) PCR melting curve
PCR熔解曲线
1.
Objective Take DNA sequencing method as the standard,to explore the sensitivity and specificity of HBV-YMDD mutation by PCR melting curve method.
目的:以DNA测序法为标准,探讨PCR熔解曲线法检测乙型肝炎病毒YMDD基因变异的敏感性和特异性。
3) FH-PCR-MC
熔解曲线法
1.
Methods The HBV YMDD mutation was detected by fluorescent hybridization double probe PCR and melting curve assay(FH-PCR-MC) in 119 patients with CHB who were not treated with lamivudine and in 30 patients with CHB who were treated with lamivudine.
方法选择未接受过抗病毒治疗的CHB患者119例和经拉米夫定治疗后无明显效果的CHB患者30例,采用荧光标记杂交双探针聚合酶链反应熔解曲线法(FH-PCR-MC)对其血浆标本进行YMDD基因序列突变检测。
4) melting curve analysis
熔解曲线分析
1.
Rapid detection of GSTM1 polymorphisms using SYBR green I and melting curve analysis;
SYBR greenⅠ结合熔解曲线分析快速检测GSTM1基因多态性
2.
Detection of mtDNA A1555G mutation by allele-specific PCR and its melting curve analysis
等位基因特异PCR结合熔解曲线分析快速检测线粒体DNA A1555G突变
3.
Detection of RSV A and B were based on determination of specific melting temperatures by real-time PCR and melting curve analysis using SYBR GreenⅠfluorescent dye.
使用SYBR Green荧光染料结合熔解曲线分析进行检测,根据产物特征性熔解温度(melting temperatures Tm)鉴别RSV A、B亚型。
5) Tm-shift curve
熔解温度曲线
1.
Here we will describe new methods such as denaturing high performance liquid chromatography(DHPLC),primer extension and time-of-flight mass spectrometry(TOF-MS),Tm-shift curve,allele-specific amplification-Tm shift,Molecular beacons and TaqMan.
当前,已建立了许多SNP分析方法,本文介绍变性高压液相色谱法、时间飞行质谱熔解曲线法、熔解温度曲线法、等位基因特异扩增结合熔解曲线法、分子信号和TaqMan等新的SNP分析方法。
6) High resolution melting
高分辨率熔解曲线
1.
High resolution melting analysis of small amplicons and of mixing with a known genotype for genotyping of mitochondrial SNP13928G > C
高分辨率熔解曲线小扩增子法结合混样法在线粒体13928G>C突变分析中的应用
2.
High resolution melting analysis which is based on the physical properties of nucleic acid for genotyping or mutation scanning of PCR products by using of high resolution instruments with special "saturation"dyes without special probes has recently been reported.
高分辨率熔解曲线是根据核酸的特性,无需使用序列特异性探针,利用一种饱和染料,对PCR反应产物进行基因分析的一种技术。
补充资料：Hesse曲线(代数曲线的)
Hesse曲线(代数曲线的)
Hessian (algebraic curve)
11油限曲线(代数曲线的)【H台自11(.妙如允.抖e);recc咖，T~aaa，即r药pa一吸ee二o‘二p.助蓝] n次代数曲线(司罗玩水c~)的He丈祀曲线就是其极二次曲线能分裂为两条直线的点的集合，也是第一极曲线的二重点构成的集合.n次非奇异曲线的He丈七曲线是一条次数为3伪一2)、类为3(n一2)(3n一7)的曲线.设介O是这条n次曲线的齐次坐标方程，关丘=刁:f/刁xi刁、，则它的He丈犯曲线的定义方程为 !不:关:五，} }五:关:五31=0. }人，人2人3}特征不等于3时的三次非奇异曲线的H既七曲线与这条曲线交于9个通常拐点.因O.H改e(l 844)而得名. A .E.H困阳。B撰
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条