1) Metal chelate chromatography
金属螯合层析
2) Ni-chelating-Sepharose
金属螯合层析
1.
After denaturation and renaturation,the fusion proteins were purified using three chromatographic columns—namely Ni-chelating-Sepharose before and after enzyme digestion,and Sephadex G-50—were operated in series.
2 mmol/L氧化型谷胱甘肽脉冲加样稀释复性;金属螯合层析收集0。
3) Metal immobilization affinity chromatography
镍金属螯合层析
4) Immobilized metal ion affinity chromatography
金属螯合亲和层析
1.
Purification of recombinant hepcidin fusion protein by immobilized metal ion affinity chromatography;
重组Hepcidin融合蛋白的金属螯合亲和层析纯化
2.
Purification of anti-HBcAg monoclonal antibodies using immobilized metal ion affinity chromatography
金属螯合亲和层析法纯化抗乙肝核心抗原单克隆抗体
3.
Immobilized metal ion affinity chromatography(IMAC) is one of the most efficient techniques in biological macromolecular separation and purification which has the advantages of high specificity, high recovery efficiency, high purity and single-step operation.
金属螯合亲和层析具有螯合介质制备简单,吸附容量大,选择性及通用性较好,易于再生,成本低等优点,是分离纯化蛋白质等生物工程产品最有效的技术之一。
5) metal chelate affinity chromatography
金属螯合亲和层析
1.
Superoxide dismutase(SOD) was preliminarily separated from maize by precipitation with ammonium sulfate and gelfiltration and then purified by metal chelate affinity chromatography(MCAC).
利用硫酸铵分级沉淀、凝胶过滤初步分离了玉米SOD,利用金属螯合亲和层析纯化了SOD。
2.
Isolation and purification of two SOD components(A and B) from maize by metal chelate affinity chromatography and DEAE-cellulose ion-exchange chromatography and determination of their qualities had been carried out in this experiment.
利用金属螯合亲和层析和DEAE-纤维素离子交换层析相结合的方法分离纯化玉米SOD,得到2种达到电泳纯的SOD组分(A、B),并对它们的基本性质进行了初步分析。
6) Immobilized metal ion affinity chromatograph(IMAC)
金属螯合亲和层析
1.
By means of Immobilized metal ion affinity chromatograph(IMAC),comparing the results of different elution methods,a optimum purification route was attained.
采用金属螯合亲和层析法对十聚体脑钠素进行了纯化 ,比较了咪唑洗脱法和降pH值洗脱法对目标蛋白的纯化效果 ,得到了一条纯化目标蛋白最佳亲和层析纯化路线 ,为后续实验打下了很好的基
补充资料:金属螯合物
分子式:
分子量:
CAS号:
性质:螯合剂与金属形成的化合物。β-乙酰酮与二价金属形成的化合物(如图)。其中乙酰丙酮为螯合剂。大多数螯合剂是含有N、O或S原子配位基的化合物,典型的螯合剂有乙二胺、二亚乙基三胺、乙二胺四乙酸(EDTA)等。螯合剂用途很广,EDTA可用作软水剂、锅炉除垢剂,三聚磷酸盐螯合剂添加到合成洗涤剂中可以螯合硬水中的钙、镁离子等。
分子量:
CAS号:
性质:螯合剂与金属形成的化合物。β-乙酰酮与二价金属形成的化合物(如图)。其中乙酰丙酮为螯合剂。大多数螯合剂是含有N、O或S原子配位基的化合物,典型的螯合剂有乙二胺、二亚乙基三胺、乙二胺四乙酸(EDTA)等。螯合剂用途很广,EDTA可用作软水剂、锅炉除垢剂,三聚磷酸盐螯合剂添加到合成洗涤剂中可以螯合硬水中的钙、镁离子等。
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
参考词条