1)  SYBR Green I
SYBR Green I
1.
Establishment of methods for detection of dengue virus by real-time quantitative RT-PCR with SYBR Green I.;
SYBR Green I荧光PCR检测登革热病毒基因方法的建立
2.
Combination of SYBR Green I and TaqMan Probe in the Detection of HBV-DNA by Fluorescent Quantitative PCR;
SYBR Green I联合TaqMan荧光定量PCR检测HBV-DNA的意义
3.
Quantification of Simian Immunodeficiency Virus(SIV) Viral Load Using Real-Time Quantitative RT-PCR with SYBR Green I;
SYBR Green I实时荧光定量RT-PCR测定猴免疫缺陷病毒RNA拷贝数方法的建立
2)  SYBR Green I
SYBRGreenI
1.
Objective: To establish SYBR GREEN I real-time RT PCR method with the external standard to detect the expression of TSHR mRNA in thyroid in the mice immunized with guinea pig TSHR.
目的:建立外标准法SYBRGREENI实时定量(real-time)RT-PCR方法,以检测豚鼠TSHR分子免疫对BALB/c小鼠甲状腺TSHRmRNA的表达的影响。
2.
SYBR Green I is widely used in real-time PCR applications as an intercalating dye.
SYBRGreenI是一种在实时定量PCR检测中广泛使用的DNA结合染料。
3)  SYBR Green I
SYBR GreenI
1.
Standard curve was established using a series dilution of quantified plasmids to measure EZH2 using SYBR Green I real-time fluorescent polymerase chain reaction and the characteristic of specific EZH2 amplicon was analysed by melti.
目的:建立SYBR GreenI实时荧光PCR定量检测人类EZH2基因的方法。
2.
According to the avian reticuloendotheliosis virus(REV) LTR sequence available in GenBank,a pair of specific primers were designed which target to the conserved region of LTR,and SYBR Green I-based real-time PCR was developed.
根据禽网状内皮组织增生症病毒(REV)长末端重复序列LTR基因保守区域设计并合成一对引物,建立基于SYBR GreenI模式的实时荧光PCR方法(Real-timePCR)。
4)  SYBR Green I
SYB RGreen I
5)  SYBR Green I method
SYBR GreenI法
6)  SYBR GREEN I
绿色荧光染料Ⅰ
1.
Objective To examine the feasibility and practicability of quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) with SYBR GREEN I fluorescence for detecting the MYCN mRNA expression in neuroblastoma cell line LA-N-5.
目的MYCN基因表达对神经母细胞瘤的治疗及预后评估有指导意义,目前国内对于MYCN基因mRNA的定量检测未见报道,该研究拟采用SYBR绿色荧光染料Ⅰ(SYBRGREENⅠ)实时检测的逆转录-聚合酶链反应(RT-PCR)方法,检测神经母细胞瘤细胞系LA-N-5细胞MYCN基因mRNA的表达,并对其可行性及实用性进行研究,力争探索出微量瘤标本的MYCN基因mRNA定量检测的可行方法。
参考词条
补充资料:Green oil
分子式:C14H10
分子量:178.22
CAS号:120-12-7

性质:带有淡蓝色荧光的针状晶体。具有半导体的性质。熔点为218℃,沸点340℃,226.5℃(7.07kPa),闪点(闭口)121.11℃,相对密度1.25(27/4℃),1.252(25/4℃),自燃点472℃,粘度(222.3℃)50.1mPa·s,折射率1.5948。不溶于水,微溶于醇、醚,能溶于苯,氯仿和二硫化碳。性质比较活泼,在空气中,日光下易被氧化,加热时升华。其粉尘与空气混合能爆炸,爆炸极限为5.04g/m3。

制备方法:在煤焦油中,蒽的含量为1.4%。焦油连续蒸馏所得的I蒽油馏分段,占焦油12-14%,初馏点为300℃,在350℃前的馏出量为95%。I蒽油经冷却结晶、真空过滤或用离心机脱除部分油分,离心后的粗蒽含油分大于20-25%,其余成分是:蒽25-30%,咔唑20-25%、菲30%左右。其中蒽的收率占焦油中蒽源的58-87%。除了苯和萘以外,蒽是煤焦油中最重要的组分之一。在蒽的工业生产中,因为必须把沸点相近的菲和咔唑分离掉,所以比萘的制造困难得多。即使把蒸馏、结晶及溶剂抽提巧妙地结合起来,其产率也只有40-50%。从粗蒽中提取和精制蒽,是先利用菲易溶于油的特性使菲与蒽、咔唑分离。进一步分离蒽与咔唑的方法有蒸馏分离法和钾熔法。

用途:本品主要用于制造染料中间体蒽醌及单宁。可用作杀虫剂、杀菌剂和汽油阻凝剂。高纯蒽用于制取单晶蒽,用在闪烁计数器上。

说明:补充资料仅用于学习参考,请勿用于其它任何用途。