1) reverse transcription
逆转录
1.
Process of HIV-1 reverse transcription and its detection by using PCR;
HIV-1逆转录过程及应用PCR检测
2.
The germline mutations of MLH1 and MSH2 mRNA were detected by cDNA sequencing analysis following reverse transcription-PCR(RT-PCR) with special primers, heat-resistance reverse transcriptase, and expand long template PCR.
方法收集符合Amsterdam标准Ⅱ的12个家系14名家庭成员外周血,用特异引物和耐热性逆转录酶特异地逆转录MLH1和MSH2的RNA;利用长模板PCR扩增酶扩增逆转录产物(cDNA);测序分析扩增产物。
3.
The process of reverse transcription can be divided into several steps:formation of minus-strand strong-stop DNA;the first translocation; initiation of plus-strand DNA synthesis;the second translocation and the completion of both strands.
人类免疫缺陷病毒(Human Immunodeficiency Virus,HIV)是逆转录病毒,进入宿主细胞胞浆即以其正链RNA基因组为模板,在逆转录酶的作用下开始逆转录合成双链的DNA。
2) Reverse transcript
逆转录
1.
Methods We used reverse transcript,nested PCR and southern hybridization with α fetoprotein mRNA which can be symbolized in hepatocellular carcinoma(HCC).
方法 采用逆转录和巢式多聚酶链式反应及Southern杂交技术 ,将AFPmRNA作为肝癌细胞的特异标志 ,特异扩增检测肝癌患者外周血中微量转移的肝癌细胞。
3) RT-PCR
逆转录PCR
1.
Cloning and Sequencing of Tumor Suppressor Gene LKB1/STK11 Associated with Peutz-Jeghers Syndrome by RT-PCR;
PJ综合症相关抑癌基因LKB1/STK11的逆转录PCR法克隆及序列分析
2.
Methods Expression of PLC-epsilon in bladder tissues of 27 patients with TCCB and 12 normal bladder tissues was detected by RT-PCR,and its relationship with clinicopathological characteristics of tumor was analyzed.
方法采用逆转录PCR(RT-PCR)方法检测27例膀胱移行细胞癌和12例正常膀胱对照组中PLCε mRNA表达,分析其与膀胱病理学特征的关系。
3.
Me thods Total RNA was isolated from rat brain tissue, and the coding region of DNA polymerase β was amplified by reverse transcriptase - polymerase chain reaction (RT-PCR).
方法提取大鼠脑组织总RNA,用逆转录PCR扩增DNA多聚酶β(POLB)的编码区,与T载体连接,作DNA测序后,酶切POLB基因并连入腺病毒穿梭质粒pAdTrack-CMV,电转至含腺病毒pAdeasy骨架质粒的大肠杆菌BJ5183。
4) reverse transcriptase
逆转录酶
1.
RT-PCR kit with different reverse transcriptase affected detecting result of COX-2 mRNA expressoin;
不同逆转录酶的RT-PCR反应体系影响COX-2 mRNA表达的检测结果
2.
Progress in HIV non-nucleoside reverse transcriptase inhibitors (NNRTIs);
HIV非核苷类逆转录酶抑制剂研究进展
3.
Expression of telomerase reverse transcriptase and its significance in human fetal lung development;
人胎肺发育不同阶段端粒酶逆转录酶的表达及意义
5) reverse transcription polymerase chain reaction
逆转录PCR
1.
Methods The expression of SSTR subtype 1～5 mRNA was determined by reverse transcription polymerase chain reaction method in 30 cases of pancreatic cancer and in 12 cases of normal pancreas tissues.
方法应用逆转录PCR(RT-PCR)检测30例胰腺癌组织和12例正常胰腺组织生长抑素受体亚型mRNA的表达。
6) RT-PCR
逆转录-PCR
1.
The expression of multidrug resistance(mdr 1) gene was tested by RT-PCR and Western blot.
方法采用MTT法检测X射线照射后CNE-1细胞对顺铂(cD-DP)的耐药性;采用逆转录-PCR(RT-PCR)和蛋白免疫印迹(W estern b lot)方法,检测照射后CNE-1细胞mdrl基因的表达。
补充资料：逆转录
分子式：
CAS号：
性质：又称逆转录。以RNA为模板产生DNA分子的过程。
CAS号：
性质：又称逆转录。以RNA为模板产生DNA分子的过程。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条