1) reverse transcription at high temperature
高温逆转录
2) high-retroviral
高效逆转录
3) reverse transcription loop-mediated isothermal amplification (RT-LAMP)
环介导逆转录等温扩增
4) RT-LAMP
逆转录环介导等温扩增
1.
To develop a reverse transcription-loop-mediated isothermal amplification(RT-LAMP) assay for rapid and sensitive detection of Norwalk GⅡ.
建立一种检测Norwalk病毒GⅡ型快速、敏感的一步逆转录环介导等温扩增(Reverse transcription-loop-me-diated isothermal amplification,RT-LAMP)方法。
5) RT-PCR
逆转录PCR
1.
Cloning and Sequencing of Tumor Suppressor Gene LKB1/STK11 Associated with Peutz-Jeghers Syndrome by RT-PCR;
PJ综合症相关抑癌基因LKB1/STK11的逆转录PCR法克隆及序列分析
2.
Methods Expression of PLC-epsilon in bladder tissues of 27 patients with TCCB and 12 normal bladder tissues was detected by RT-PCR,and its relationship with clinicopathological characteristics of tumor was analyzed.
方法采用逆转录PCR(RT-PCR)方法检测27例膀胱移行细胞癌和12例正常膀胱对照组中PLCε mRNA表达,分析其与膀胱病理学特征的关系。
3.
Me thods Total RNA was isolated from rat brain tissue, and the coding region of DNA polymerase β was amplified by reverse transcriptase - polymerase chain reaction (RT-PCR).
方法提取大鼠脑组织总RNA,用逆转录PCR扩增DNA多聚酶β(POLB)的编码区,与T载体连接,作DNA测序后,酶切POLB基因并连入腺病毒穿梭质粒pAdTrack-CMV,电转至含腺病毒pAdeasy骨架质粒的大肠杆菌BJ5183。
6) reverse transcription
逆转录
1.
Process of HIV-1 reverse transcription and its detection by using PCR;
HIV-1逆转录过程及应用PCR检测
2.
The germline mutations of MLH1 and MSH2 mRNA were detected by cDNA sequencing analysis following reverse transcription-PCR(RT-PCR) with special primers, heat-resistance reverse transcriptase, and expand long template PCR.
方法收集符合Amsterdam标准Ⅱ的12个家系14名家庭成员外周血,用特异引物和耐热性逆转录酶特异地逆转录MLH1和MSH2的RNA;利用长模板PCR扩增酶扩增逆转录产物(cDNA);测序分析扩增产物。
3.
The process of reverse transcription can be divided into several steps:formation of minus-strand strong-stop DNA;the first translocation; initiation of plus-strand DNA synthesis;the second translocation and the completion of both strands.
人类免疫缺陷病毒(Human Immunodeficiency Virus,HIV)是逆转录病毒,进入宿主细胞胞浆即以其正链RNA基因组为模板,在逆转录酶的作用下开始逆转录合成双链的DNA。
补充资料：逆转录
分子式：
CAS号：
性质：又称逆转录。以RNA为模板产生DNA分子的过程。
CAS号：
性质：又称逆转录。以RNA为模板产生DNA分子的过程。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条