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1)  3T3-L_1 cells
3T3-L_1细胞
1.
Effect of different glucose concentrations on the expressions of insig-1 and insig-2 mRNA during the differentiation of 3T3-L_1 cells;
不同浓度葡萄糖对3T3-L_1细胞分化及insig-1和insig-2 mRNA表达的影响
2)  3T3-L1 cells
3T3-L1细胞
1.
The role of galanin in resistin gene expression in rat adipose tissue and 3T3-L1 cells;
在大鼠脂肪组织及3T3-L1细胞中甘丙肽对抵抗素基因表达的影响
2.
Methods The cultured 3T3-L1 cells were pretreated with naringin,and then MTT method was used to detect the proliferation of the cells,oil red O staining method and spectrophotography were applied to analyze the degree of differentiation.
方法培养3T3-L1细胞,并用不同浓度的柚皮苷进行干预,以四甲基偶氮唑盐(MTT)法检测细胞增殖,用油红O染色和染色比色法分析脂肪细胞的分化程度。
3)  3T3-L1
3T3-L1细胞
1.
Meanwhile,3T3-L1 cells were used and incubated respectively with arachid.
同时进行了PUFAs对体外培养3T3-L1细胞分化的干预实验,在细胞被诱导分化D5,在培养基中分别加入油酸(OA,C18:1n-9)、花生四烯酸(AA,C20:4n-6)、二十碳五烯酸(EPA,C20:5n-3)和二十二碳六烯酸(DHA,22:6n-3,0。
4)  3T3-L1 cell
3T3-L1细胞
1.
Insulin up-regulates expression of apelin gene in 3T3-L1 cells:a preliminary observation;
胰岛素上调3T3-L1细胞apelin基因表达的初步观察
2.
Effects of recombinant adenovirus-mediated siRNA on adiponectin expression in 3T3-L1 cells;
腺病毒载体介导的siRNA对3T3-L1细胞脂联素表达的影响
3.
Ten novel compounds were designed and synthesized on the basis of compound 1,their insulin-sensitizing activities were evaluated in 3T3-L1 cells.
结果表明化合物10在3T3-L1脂肪细胞模型上表现出较强的促3T3-L1细胞分化活性,提示其可能具有较好的胰岛素增敏作用。
5)  NIH 3T3 cells
NIH 3T3细胞
1.
Construction of pLNCX/anti-CD20scFv/IgGFc/CD80/CD28/ζ eukaryotic expression vector and expression in NIH 3T3 cells;
真核表达载体pLNCX/anti-CD20scFv/IgGFc/CD80/CD28/ζ的构建及其在NIH 3T3细胞株中的表达
2.
Objective To establish a cytoplasmic M-CSF-stably-expressing cell line, and to explore the effect of cytoplasmic M-CSF on the proliferation and mobility of NIH 3T3 cells.
方法:PCR分别扩增人M-CSF的胞外活性区和功能区,连接到胞浆定位载体pCMV/cyto/myc质粒,得重组质粒pCMV/cyto/myc-h-M-CSF,再将重组质粒稳定转染NIH 3T3细胞,得到分别稳定表达M-CSF的胞外活性区和功能区的细胞株。
6)  NIH 3T3 cell
NIH 3T3细胞
1.
To investigate the expression of Nogo-A and srGAPs in NIH 3T3 cells, Western blot analysis was performed to verify protein expression of Nogo-A and demonstrated specific Nogo-A bands at about 230 kD on NIH 3T3 cell extracts.
为检测Nogo-A和srGAPs蛋白在NIH 3T3细胞上的表达,应用Western印迹的方法检测Nogo-A蛋白的表达。
2.
Methods Plasmid containing the human PDGFR-β gene was constructed and stably transfected into the mouse NIH 3T3 cells,followed by the functional analysis of the transfected clone cells.
1-PDGFR-β,将其转染至NIH 3T3细胞,获得稳定转染的细胞克隆,并对其进行功能学鉴定及应用;应用瞬时转染PDGFR-β的HeLa细胞,建立PDGF依赖性的受体磷酸化细胞模型。
补充资料:“痘痕”红细胞


“痘痕”红细胞
“pocked”red blood cell

  Koyama及其同道(1962年)首先利用扫描电镜发现切除脾脏患者,有相当比例的红细胞表面显示“空泡”,而正常人有此空泡者少见。Holroyd(1969年)等利用干涉显微镜证实无脾者的许多红细胞的光滑表面出现“凹陷”,形态似“痘痕”,故称为“痘痕”红细胞。此种“痘痕”即扫描电镜下所见的“空泡”。用干涉显微镜计数循环血“痘痕”红细胞百分数,可评价脾功能。将脾功能分3种状态:正常脾功能的痘痕红细胞值为0~0.3%,无脾功能测值为19%~52%,脾功能低下的新生儿测值为0.6%~2.8%。“痘痕”的检测对评价脾功能及进一步开展脾移植和保留脾脏手术的临床实践和科研,是一项有用的、简便有效方法。
  
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