1) Western-blot
Western-blot
1.
Protein SD32 antigen-specific analyzed by Western-blot;
Western-blot法测定重组人纽表位肽12的抗原特异性
2.
anthropophagus midgut crude and membrane protein(harvested by purification from crude protein) by Western-blot.
采用Western-blot免疫学方法对嗜人按蚊中肠粗蛋白和膜蛋白(即纯化后的粗蛋白)分子量及免疫原性进行分析。
3.
The method of SDS-PAGE and Western-blot for isolating and Identifying the specific protein of Mark s virus.
为快速诊断此病,建立了一种应用SDS-PAGE电泳和Western-blot染色分离鉴定、纯化马立克氏病毒特异性抗原蛋白的方法,并获得了与MDV抗血清特异反应的抗原蛋白。
2) Western-blot
Western-blot法
1.
The protein levels of SOCS-3 were further analysed by Western-blot.
方法实时荧光定量PCR法检测60例脑梗死患者和40例健康对照者的PBMC中SOCS-3 mRNA表达,Western-blot法检测其蛋白质水平的表达。
2.
Methods:An immunohistochemical analysis with antibodies to eIF4E was performed on Fifty-two specimens of the LSCC,Twenty specimens of dysplasia,Thirty specimens of hyperplasia and ten specimens of normal mucosa;Western-blot analysis was performed on tumor core,transition core,tumor-free core of twenty specimens of fresh LSCC using anti-eIF4E,antiserums.
方法:采用免疫组织化学法检测52例蜡块LSCC组织、20例喉黏膜不典型增生组织、30例喉良性增生组织及10例正常喉黏膜组织中eIF4E基因蛋白的表达情况;用Western-blot法检测20例新鲜LSCC标本的癌中心区(TC)、过渡区(TR)及病理证实的无癌区(TF)中eIF4E的表达情况。
3.
Material 40 cases of tissues of gastric cancer and 40 cases of normal tissues were collected, in accordance withβ-actin, and the expression of TSC1 protein were detected by Western-Blot.
方法收集胃癌组织标本40例,癌旁正常组织标本40例,用Western-Blot法检测样本中TSC1蛋白和β-actin蛋白含量,分别测定TSC1带与β-actin带的黑度值,以黑度值的比值A值表示TSC1蛋白表达水平。
3) Western-blot
免疫印记
1.
Assessment,purification and identification of Taenia solium cysticercus cyst fluid angen by two-dimentional electrophoresis and Western-blot.;
双向电泳及免疫印记法对囊尾蚴囊液抗原的分析、纯化及鉴定
4) Western-blot
Western-blot技术
1.
Methods:RT-PCR and Western-blot were used to identify the expression of LRIG2 mRNA and protein in 38 bladder cancer tissue and 16 normal bladder epithelium tissue.
方法:采用逆转录-聚合酶链反应和Western-blot技术检测了38例膀胱癌组织和16例正常膀胱黏膜组织中LRIG2mRNA和蛋白的表达情况。
5) Western blot
Western blot
1.
An analysis of Western blot for detection of B7-2 protein in liver of patient with hepatic cirrhosis;
B7-2分子在肝硬化患者肝脏组织中表达的Western blot检测方法研究
2.
Western blot was used to detect the changes of protein level of DPPII in the lens of eac.
方法:建立D-半乳糖性白内障大鼠动物模型,进行相应分期分组,应用Western blot方法检测DPPII在各组白内障大鼠及正常大鼠晶状体中含量变化。
3.
Methods: Rat skeletal muscular proteins were separated and transferred to the nitrocellulose membrane by using a combining method of two-dimensional electrophoresis and Western blot.
方法:用双向电泳结合Western blot方法将分离的大鼠骨骼肌蛋白转移至硝酸纤维膜,利用镜像原理,识别与CAⅢ抗体发生免疫反应的抗原在双向电泳图谱中的确切位置。
6) Western Blot
免疫印记法
1.
Objective:()To investigate the clinical significance of human papillomavirus(HPV)16,18E6 infection in cervical liquid-based cytology residual cells from patients with cervical intraepithelial neoplasis(CIN) by Western blot.
目的:探讨免疫印记法(Western blot)检测子宫颈上皮内瘤变(cervical intraepithelial neoplasis,CIN)宫颈液基细胞残液中人乳头状瘤病毒(human papillomaviruses,HPV)16、18E6感染的临床意义。
参考词条
Western blot
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防伪胶粘封记
C.I.酸性绿81
补充资料:Western blotting
分子式:
CAS号:
性质:一种类似于DNA斑迹法,用于从凝胶中转移蛋白质的技术。转移到膜上的蛋白质用同位素或酶标记过的抗体显示目的带的存在。
CAS号:
性质:一种类似于DNA斑迹法,用于从凝胶中转移蛋白质的技术。转移到膜上的蛋白质用同位素或酶标记过的抗体显示目的带的存在。
说明:补充资料仅用于学习参考,请勿用于其它任何用途。