1) In situ hybridization
原位杂交技术
1.
In situ hybridization and Streptavidin-peroxidase(S-P) immunohistochemical method were used to detect the expr.
建立大鼠心肌缺血再灌注损伤模型,测定SOD、GSH-Px活性,应用原位杂交技术和免疫组化S-P法检测HSP70蛋白的表达。
2.
Methods Using in situ hybridization to detect the expressions of Cbfa1 in the synovial tissues of the patients cacroiliac joint with active AS,comparing the results with those in the patients with pelvic fracture and inactive AS;using image analysis to analyze the pictures.
方法通过原位杂交技术检测活动期AS患者与对照组(骨盆骨折患者以及静止期AS患者)骶髂关节滑膜组织中Cbfa1的表达情况,用图像分析系统比较它们的表达差异。
3.
In situ hybridization assay and immunohistochemical S-P method were used to detect the expression of HSP70 mRNA and its protein in 30 c.
建立大鼠心肌缺血再灌注损伤模型,应用原位杂交技术和免疫组化S-P法检测HSP70mRNA及其蛋白的表达。
2) fluorescence in situ hybridization
荧光原位杂交技术
1.
ZNF217 gene was detected in ovarian serous cystadenocarcinoma by fluorescence in situ hybridization;
应用荧光原位杂交技术检测卵巢浆液性囊腺癌中ZNF217基因拷贝数
2.
Localization of Human β~E-globin Gene in Chromosomes of Transgenic Mice by Fluorescence in situ Hybridization;
应用荧光原位杂交技术检测人β~E珠蛋白基因在转基因小鼠染色体上的整合状态
3.
Application of Fluorescence in situ Hybridization in Botany;
荧光原位杂交技术在植物学中的应用
3) FISH
[英][fɪʃ] [美][fɪʃ]
荧光原位杂交技术
1.
Detection of Chromosome 6 Aberration in Lung Carcinoma Using Fluorescence in Situ Hybridization(FISH);
荧光原位杂交技术检测肺癌第6号染色体畸变
2.
Furthermore,the key factor of achieving and stabilizing partial nitrification for a long time was optimization of nitrifying microbial population,which was proved by FISH analysis.
荧光原位杂交技术(FISH)检测结果证实,SBBR中短程硝化的实现与稳定是因为菌群得到了优化,氨氧化细菌成为优势菌种。
4) in situ hybridization histochemistry(ISHH)
组织原位杂交技术
1.
Methods The expression was detected by in situ hybridization histochemistry(ISHH) using labeled digoxigenin probes in the gastric cancer and normal tissues.
方法 利用随机引物法将新片段用地高辛标记为探针 ,通过组织原位杂交技术 (Insituhybridizationhistochemistry ,ISHH)检测其在胃癌及癌旁正常组织中的表达情况。
5) EM in situ hybridlzation
电镜原位杂交技术
6) nucleic acid hybridization in situ
核酸原位杂交技术
补充资料：荧光原位杂交
荧光原位杂交
是以荧光素标记取代核素标记而形成的一种新的原位杂交方法。利用已知碱基序列的非核素标记的核酸探针，依据碱基配对原理，通过免疫细胞化学检测体系，在组织切片、细胞间期核或染色体等标本上，进行DNA定性、定位及定量分析。本法快速、安全、灵敏度高，特异性强。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条