METHODS Neuron injuring model was produced by using high air pressure and assessed by nuclear uptake of the fluorescent dye propidium iodide (PI), which is excluded from cell with intact membranes however the dye penetrates injured cells and binds to DNA in the nuclei which then fluo resces red.

Then the effector cells were conserved in 4℃ and 25℃ condition,using propidium iodide (PI) to dye the effector cells and using flow cytometer to detect the survival rate of effector cells in this two different conditions in different time.

The iodination of acetone experiment software developed by Delphi 6.0 and the object Pascal language;

To evaluate the method of measuring apoptosis by flow cytometry with three stains, the apoptosis percentage of rat cortical neurons was measured by flow cytometry using propidium iodide(PI) staining, Annexin V/PI and JC-1 double staining respectively.

In this paper, to solve the pollution problem in water, the applicability of electro catalytic oxidation on killing algae and microcystin-LR removal was examined using Ti/RuO_2 as anode, at the same time explored the viability determination of blue-green algae by FDA-PI double stai.