1) RNA-dependent RNA polymerase
RNA依赖的RNA聚合酶
1.
Yeast expression plasmid containing hepatitis C virus nonstructural gene 5b from infection clone was constructed and transformed into Pichia pastori,constitutive expression of soluble HCV RNA-dependent RNA polymerase(RdRp)was obtained and indicated to be special 64.
构建含有感染性克隆HCV非结构基因 5b区序列的酵母表达质粒 ,转化毕加酵母 ,获得持续、可溶性HCVRNA依赖的RNA聚合酶 (RdRp)的表达 ,纯化蛋白在SDS -PAGE及Westernblot中显示出特异性的 6 4 2kDHCVRdRp蛋白带 ,同聚引物 /模板测定显示RdRp的活性极低 ,延长反应时间 ,RNA聚合活性仅轻度升高。
2.
The 3D gene encoding RNA-dependent RNA polymerase(RdRp) of foot-and-mouth disease virus was amplified by RT-PCR using pfu ultra~(TM) High-Fidelity DNA polymerase,and the amplicon was cloned into the eukaryotic expression vector pcDNA3.
利用RT-PCR技术扩增口蹄疫病毒(FMDV)编码RNA依赖的RNA聚合酶的3D基因,经测序确认后将其克隆到真核表达质粒载体pcDNA3。
3.
The full-length NS5 (NS5F) and the NS5 fragment encoding the RdRp, RNA-dependent RNA polymerase, domain (NS5pol) were PCR amplified from a full-length WNV cDNA clone and cloned into the prokaryotic expression vectors pET-28a, respectively.
以病毒全长cDNA克隆为模板,PCR扩增获得NS5的RNA依赖的RNA聚合酶(RdRp)活性区(NS5pol)及该蛋白完整的编码序列(NS5F),分别克隆于原核表达载体pET-28a并转化至大肠杆菌E。
2) RNA-dependent RNA polymerase
依赖于RNA的RNA聚合酶
1.
In the structural proteins, the P1 was the putative RNA-dependent RNA polymerase, which might play an important role in the synthesis of RGDV genomic dsRNAs;The P5 bound with GTP and UTP was a NTP binding protein with RNA guanylyltransferase activity that synthesized a cap structure (mGpppAmpGp) for.
采用α 32 P标记的三磷酸核苷酸与病毒粒子共浴的方法及Northwestern分子杂交方法 ,研究证明了RGDV结构蛋白中 ,P1蛋白可能是依赖于RNA的RNA聚合酶 ,P5蛋白可能具有RNA鸟苷酰转移酶功能 ,P6蛋白可能是核酸结合蛋白 ,是RGDV复制包装中的关键蛋白。
3) RNA-dependent RNA polymerase
依赖RNA的RNA聚合酶
1.
The RNA-dependent RNA polymerase(RdRp) is the viral replicase.
正链RNA病毒的基因组为正链RNA,其复制酶是依赖RNA的RNA聚合酶,非编码区是病毒基因组复制的主要调控位点,3’非编码区是复制酶的第一结合位点,正链RNA病毒基因组大多可能按copy-back模型进行复制。
4) RNA-dependent RNA polymerase(RdRp)
依赖于RNA的RNA聚合酶
1.
Cloning of RNA-dependent RNA polymerase(RdRp) gene of HCV and its expression in E.coli system;
丙型肝炎病毒依赖于RNA的RNA聚合酶基因克隆与原核表达
5) RNA dependent RNA polymerase(RdRp)
依赖RNA的RNA聚合酶(RdRp)
6) NS5B RNA-dependent RNA polymerase
NS5B RNA依赖的RNA聚合酶
1.
Hepatitis C virus (HCV) NS3/4A serine protease and NS5B RNA-dependent RNA polymerase are vital for the processing and maturation of viral protein precursors and RNA replication.
丙型肝炎病毒(hepatitis C virus,HCV)NS3/4A丝氨酸蛋白酶和NS5B RNA依赖的RNA聚合酶是病毒蛋白前体加工成熟和复制过程中十分重要的两个酶,是抗HCV治疗的理想靶点。
补充资料：RNA
见核糖核酸。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条