2) glycerol-3-phosphate dehydrogenase
胞浆3-磷酸甘油脱氢酶
1.
We cloned and characterized a 4900-bp genomic fragment containing the CgGPD gene encoding a glycerol-3-phosphate dehydrogenase homologous to GPD genes in other yeasts using degenerate primers in conjunction with inverse PCR.
产甘油假丝酵母WL2002-5具有耐高渗且高产甘油的特性,本课题组已通过染色体步移法首次成功克隆了来源于产甘油假丝酵母的胞浆3-磷酸甘油脱氢酶编码基因CgGPD1,通过基因序列比对,发现该基因是全新的基因,有关该酶的酶学性质的研究未见报道,为了能更好的解释该酶在高渗条件下保持高酶活和产甘油假丝酵母的耐高渗甘油合成的代谢机理,因此对该酶的酶学性质的研究显得十分迫切。
4) Glycerol-3-Phosphate dehydrogenase
3-磷酸甘油脱氢酶
1.
This paper describes the first cloning of the glycerol-3-phosphate dehydrogenase gene (DSGPDH) of Dunaliella salina involving large-scale sequencing of a cDNA library and RACE.
本文通过对盐生杜氏藻(Dunaliellasalina)cDNA文库进行大规模EST测序并结合RACE实验克隆了盐生杜氏藻3-磷酸甘油脱氢酶基因,并且通过Southernblotting实验确定了该基因在这个物种中拷贝数。
2.
Glu could clearly increase the intracellular protein content,activity of glycerol-3-phosphate dehydrogenase(GPDH),and the total amount of intracellular protein,activity of GPDH,specific activity of GPDH reached maximal values,which were 1.
葡萄糖对盐藻细胞内总蛋白、3-磷酸甘油脱氢酶(GPDH)酶活和比活都有显著影响,在15g/L葡萄糖时这3个值达到最大值,分别是对照的1。
5) Glycerol 3-phosphate dehydrogenase
3-磷酸甘油脱氢酶
1.
Therefore, function studies of glycerol 3-phosphate dehydrogenase (GPD) is important for plant stress research and glycerol-produce engineering strain construction.
依赖NAD~+辅酶的3-磷酸甘油脱氢酶(glycerol-3-phosphate dehydrogenase,GPD,EC1。
2.
First, the genes encoding glycerol 3-phosphate dehydrogenase (GPD1) and glycerol 3-phospatase (HOR2) were amplified from total DNA of Saccharomyces cerevisiae using PCR, respectively.
首先，从酿酒酵母Saccharomyces cerevisiae中克隆3-磷酸甘油脱氢酶(GPD1)和3-磷酸甘油酯酶(HOR2)基因，分别构建重组质粒pSE-gpd1和pSE-hor2，然后进行两种重组基因的表达试验，一种是双表达盒法，另一种是多顺反子法。
3.
Unlike GPDH reported from other species,the glycerol 3-phosphate dehydrogenase from D.
3-磷酸甘油脱氢酶(GPDH)是盐生杜氏藻甘油合成途径中的关键酶。
6) Glyceraldehyde-3-phosphate dehydrogenase
甘油醛-3-磷酸脱氢酶
1.
The amino acid sequence of glyceraldehyde-3-phosphate dehydrogenase(GAPDH)(AAN09371) from Drosophila melanogaster was used to tblastn searching EST of Bombyx mori.
从家蚕EST数据中检索到果蝇(Drosophila melanogaster)甘油醛-3-磷酸脱氢酶(GAPDH)(AAN09371)氨基酸同源序列,用seqMAN延伸,电子克隆家蚕gapdh的cds,用PCR克隆家蚕gapdh基因序列。
补充资料：胞浆
胞浆
〖HT5”SS〗cytoplasm
即“细胞质”。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条