2) Glycerol-3-Phosphate dehydrogenase
3-磷酸甘油脱氢酶
1.
This paper describes the first cloning of the glycerol-3-phosphate dehydrogenase gene (DSGPDH) of Dunaliella salina involving large-scale sequencing of a cDNA library and RACE.
本文通过对盐生杜氏藻(Dunaliellasalina)cDNA文库进行大规模EST测序并结合RACE实验克隆了盐生杜氏藻3-磷酸甘油脱氢酶基因,并且通过Southernblotting实验确定了该基因在这个物种中拷贝数。
2.
Glu could clearly increase the intracellular protein content,activity of glycerol-3-phosphate dehydrogenase(GPDH),and the total amount of intracellular protein,activity of GPDH,specific activity of GPDH reached maximal values,which were 1.
葡萄糖对盐藻细胞内总蛋白、3-磷酸甘油脱氢酶(GPDH)酶活和比活都有显著影响,在15g/L葡萄糖时这3个值达到最大值,分别是对照的1。
3) Glycerol 3-phosphate dehydrogenase
3-磷酸甘油脱氢酶
1.
Therefore, function studies of glycerol 3-phosphate dehydrogenase (GPD) is important for plant stress research and glycerol-produce engineering strain construction.
依赖NAD~+辅酶的3-磷酸甘油脱氢酶(glycerol-3-phosphate dehydrogenase,GPD,EC1。
2.
First, the genes encoding glycerol 3-phosphate dehydrogenase (GPD1) and glycerol 3-phospatase (HOR2) were amplified from total DNA of Saccharomyces cerevisiae using PCR, respectively.
首先，从酿酒酵母Saccharomyces cerevisiae中克隆3-磷酸甘油脱氢酶(GPD1)和3-磷酸甘油酯酶(HOR2)基因，分别构建重组质粒pSE-gpd1和pSE-hor2，然后进行两种重组基因的表达试验，一种是双表达盒法，另一种是多顺反子法。
3.
Unlike GPDH reported from other species,the glycerol 3-phosphate dehydrogenase from D.
3-磷酸甘油脱氢酶(GPDH)是盐生杜氏藻甘油合成途径中的关键酶。
5) Glyceraldehyde-3-phosphate dehydrogenase
甘油醛-3-磷酸脱氢酶
1.
The amino acid sequence of glyceraldehyde-3-phosphate dehydrogenase(GAPDH)(AAN09371) from Drosophila melanogaster was used to tblastn searching EST of Bombyx mori.
从家蚕EST数据中检索到果蝇(Drosophila melanogaster)甘油醛-3-磷酸脱氢酶(GAPDH)(AAN09371)氨基酸同源序列,用seqMAN延伸,电子克隆家蚕gapdh的cds,用PCR克隆家蚕gapdh基因序列。
6) GAPDH
3-磷酸甘油醛脱氢酶
1.
These enzymes measured were glyceraldehydes-phosphate dehydrogenase (GAPDH), glycerol-3-phosphate dehydrogenase (GDH), lactate dehydrogenase (LDH), and 3-hydroxyacyl-CoA dehydrogenase (HOAD).
对3日龄粘虫雌蛾吊飞过程中4种相关酶3-羟酰辅酶A脱氢酶(HOAD)、3-磷酸甘油醛脱氢酶(GAPDH)、3-磷酸甘油脱氢酶(GDH)和乳酸脱氢酶(LDH)的研究结果表明,在室内条件下,粘虫在吊飞过程中其能量代谢有以下特点:在吊飞的初始5min,所有与糖代谢和脂肪代谢相关的酶活性都快速升高,这段时期脂肪代谢的酶活性也完全被活化,HOAD活性明显增强;但在随后的5~60min持续吊飞期间与能量代谢有关的酶活性都有所下降,表明此时飞行活性趋于平稳。
2.
MTT assay was used to measure cell viability, flow cytometry and TUNEL assay were used to test cell apoptosis, and Western blot was used to detect the expression of GAPDH.
方法采用100nmol/L鱼藤酮作用SH-SY5Y细胞24h,建立SH-SY5Y细胞凋亡模型;再用100、200、300μmol/L不同浓度的利福平进行干预,采用MTT比色法检测细胞活性,采用流式细胞术(FCM)及原位缺口末端标记染色法(TUNEL)检测细胞凋亡,Western blot法检测前凋亡蛋白3-磷酸甘油醛脱氢酶(GAPDH)的表达水平。
补充资料：酶法甘油
分子式：
CAS号：
性质：现代的发酵甘油法。以葡萄糖、蔗糖或糖蜜为原料，在生物酶的作用下，经发酵转化成甘油，转化率85%～90%，发酵滤液中的甘油含量达7%～8%，经净化、浓缩、蒸馏、精制后可得到变通工业甘油。
CAS号：
性质：现代的发酵甘油法。以葡萄糖、蔗糖或糖蜜为原料，在生物酶的作用下，经发酵转化成甘油，转化率85%～90%，发酵滤液中的甘油含量达7%～8%，经净化、浓缩、蒸馏、精制后可得到变通工业甘油。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条