1) alkaline pectate lyase
碱性果胶裂解酶
1.
Production conditions of alkaline pectate lyase by alkaline microorganism Bacillus subtilis;
碱性果胶裂解酶摇瓶发酵条件的研究
2.
Based on time courses of the specific cell growth rate and specific alkaline pectate lyase production rate, a two-stage agitation rate control strategy was developed, in which agitation rate was controlled at 500 r/min at the first 5 h, then switched to 600 r/min afterwards.
在5L小型发酵罐上,考察了不同搅拌转速对细胞生长和碱性果胶裂解酶生产的影响。
2) Pectate lyase
碱性果胶酯裂解酶
1.
The structure gene PL from Bacillus subtilis WSHB04-02 encoding pectate lyase was amplified by PCR method.
从本研究室筛选的BacillussubtilisWSHB04-02菌株中扩增出编码碱性果胶酯裂解酶的结构基因PL,将其插入大肠杆菌分泌表达载体pET22b(+)多克隆位点,得到重组载体pET22b(+)PL。
3) alkaline pectate lyase
碱性果胶酯裂解酶
1.
Properties of alkaline pectate lyase from recombinant strain E.coli JM109(pHsh PL);
重组菌产碱性果胶酯裂解酶酶学性质研究
2.
Three factors for producing alkaline pectate lyase (PL) by recombinant strain Pichia pastoris: initial concentration of CH3OH, adding amount of CH3OH and concentration of yeast nitrogen base (YNB), were investigated.
31%,优化后碱性果胶酯裂解酶(PL)表达量达到40。
4) thermostable pectate lyase
耐热碱性果胶裂解酶
1.
Expression, purification and characterization of a thermostable pectate lyase from Thermotoga maritima
海栖热袍杆菌来源的极耐热碱性果胶裂解酶的表达、纯化及定性
5) pectin lyase
果胶裂解酶
1.
Expression of a pectin lyase A gene from Aspergillus niger in Pichia pastoris GS115
黑曲霉果胶裂解酶基因在毕赤酵母Pichia pastoris GS115中的表达
2.
Through single factor and orthogonal experiment, the conditions for the pectin lyase production by Aspergillus niger were optimized, its property was also studied.
通过单因素及正交实验对黑曲霉(Aspergillus niger)产生果胶裂解酶的发酵条件进行优化,研究了该酶的性质。
3.
Fermentation conditions for Aspergillus niger wz003 for production of high active pectin lyase was investigated in this paper.
通过对黑曲霉(Aspergillus niger)wz 003液态发酵过程的调控,制备了高活性的果胶裂解酶,并讨论了发酵过程中聚半乳糖醛酸酶活力的变化情况。
6) Pectate lyase
果胶裂解酶
1.
Activities of polygalacturonase (PG) and pectate lyase (PL) of Fusarium oxysporum f.
vasinfectum)的多聚半乳糖醛酸酶(PG)和果胶裂解酶(PL)活性明显降低。
2.
Pectinase includes the propectinase, pectin esters, pectin hydrolase and pectate lyase.
它包括原果胶酶、果胶酯类、果胶水解酶和果胶裂解酶(Pectate lyase)。
3.
And pectinase can be classified as polygalacturonase (PG), Pectinmelythesterase (PME), and pectate lyase(PL) .
而果胶酶又包括多聚半乳糖醛酸酶(PG),果胶甲基酯酶(PME),果胶裂解酶(PL)。
补充资料：果胶酶
分子式：
CAS号：
性质：催化植物细胞间质(即果胶质)水解成半乳糖醛酸和果胶酸的一类酶。按其作用方式，大致可分为三类：(1)原果胶酶(protopectinase)，可使天然果胶转变为可溶性果胶。(2)果胶聚半乳糖醛酸酶(pectin polygalacturonase；PG)，使果胶或果胶酸中α-1,4键分解，而生成半乳糖醛酸。(3)果胶甲酯水解酶(pectin methylesterase；PE)，使果胶中甲酯水解，生成果胶酸。为使果胶完全分解，需要先用PE，再用PG。在果汁澄清中，亦同时用这两种酶。灰白色粉末或中黄色液体。最适作用pH值3.5～4.5，最适作用温度40～50℃。果胶酶广泛存在于植物和微生物中，动物细胞通常不能合成这类酶。工业上主要采用曲霉菌，如黑曲霉或青霉菌等生产。用于果汁加工(澄清果汁)、橘子脱囊衣以及制造浓缩果汁、果粉和低糖果冻。也可用于加强果酒的澄清效果，提高酒的收率。还可用于麻料脱胶和木材防腐等。
CAS号：
性质：催化植物细胞间质(即果胶质)水解成半乳糖醛酸和果胶酸的一类酶。按其作用方式，大致可分为三类：(1)原果胶酶(protopectinase)，可使天然果胶转变为可溶性果胶。(2)果胶聚半乳糖醛酸酶(pectin polygalacturonase；PG)，使果胶或果胶酸中α-1,4键分解，而生成半乳糖醛酸。(3)果胶甲酯水解酶(pectin methylesterase；PE)，使果胶中甲酯水解，生成果胶酸。为使果胶完全分解，需要先用PE，再用PG。在果汁澄清中，亦同时用这两种酶。灰白色粉末或中黄色液体。最适作用pH值3.5～4.5，最适作用温度40～50℃。果胶酶广泛存在于植物和微生物中，动物细胞通常不能合成这类酶。工业上主要采用曲霉菌，如黑曲霉或青霉菌等生产。用于果汁加工(澄清果汁)、橘子脱囊衣以及制造浓缩果汁、果粉和低糖果冻。也可用于加强果酒的澄清效果，提高酒的收率。还可用于麻料脱胶和木材防腐等。
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