1)  Western blot
Western blot
1.
An analysis of Western blot for detection of B7-2 protein in liver of patient with hepatic cirrhosis;
B7-2分子在肝硬化患者肝脏组织中表达的Western blot检测方法研究
2.
Western blot was used to detect the changes of protein level of DPPII in the lens of eac.
方法:建立D-半乳糖性白内障大鼠动物模型,进行相应分期分组,应用Western blot方法检测DPPII在各组白内障大鼠及正常大鼠晶状体中含量变化。
3.
Methods: Rat skeletal muscular proteins were separated and transferred to the nitrocellulose membrane by using a combining method of two-dimensional electrophoresis and Western blot.
方法:用双向电泳结合Western blot方法将分离的大鼠骨骼肌蛋白转移至硝酸纤维膜,利用镜像原理,识别与CAⅢ抗体发生免疫反应的抗原在双向电泳图谱中的确切位置。
2)  Western blot
免疫印迹
1.
Comparison on ELISA,Electroselenium method and Western blot for detection of anti-HIV antibody;
ELISA、胶体硒和免疫印迹试验检测HIV抗体结果分析
2.
Estimation of postmortem interval by determination of troponin I using Western blot technique in human pectoralis major;
免疫印迹检测人胸大肌肌钙蛋白I推断较晚期死后经过时间
3.
Diagnosis of Bovine Spongiform Encephalopathy and Scrapie by Western blot;
免疫印迹法检测牛海绵状脑病和羊瘙痒病
3)  Western blot
免疫印迹法
1.
Study on HIV-Ab combined screening diagnostics as a substitute for Western Blot;
HIV抗体筛查试剂联合检测替代免疫印迹法的对比分析
2.
Methods: glutathione-S-transferase (GST) recombinant Zta was prepared and was used as antigen in western blot.
目的:用免疫印迹法检测鼻咽癌病人血清中抗EB病毒Zta-IgG抗体。
3.
Methods Serum samples were tested for anti-SLA/LP by indirect immunofluorescence assay (IIF) and Western blot(WB), and the positive rate was compared with those of anit-SMA, anti-AMA-M2 and anti-LKM-1.
方法　用间接免疫荧光法、免疫印迹法检测抗SLA/LP抗体,并与抗平滑肌抗体(SMA)、抗线粒体抗体(AMA M2)及抗肝肾微粒体(LKM 1)抗体之间的阳性率进行比较。
4)  Western blot
蛋白免疫印迹
1.
Western blot was used to detect the alterations of Kir6 proteins in different time courses (0, 1, 3 and 5 days after 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) injections) at different structures of brain ( the striatum and substantia nigra) in parkinsonian mice.
本研究采用蛋白免疫印迹分析(Western blot)对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱导的PD小鼠模型黑质、纹状体Kir6。
2.
Methods The expression of MGMT was tested by immunocytochemistry and Western blot.
方法利用免疫细胞化学和蛋白免疫印迹(Western blot)方法,检测X射线照射后CNE细胞株MGMT表达情况。
3.
Methods The DNA and specific antibody of human bocavirus from pharyngeal extracts, nasopharyngeal swab and serum were detected using real time PCR and Western blot.
方法采用荧光定量PCR和蛋白免疫印迹技术检测患者鼻咽部抽取物、咽拭子和血清标本中的人博卡病毒DNA及特异性抗体,并全面分析感染患儿的各种临床症状、体征及常规实验室检测,同时做远期随访。
5)  Western blot
蛋白质印迹
1.
The expression of FATP4 genes in fatty liver rats was examined by semi-quantitative reverse transcription and polymerase chain reaction (RT-PCR) and Western blotting.
方法　建立高脂饮食脂肪肝模型 ,用半定量逆转录聚合酶链反应 (reversetranslationpolymerasechainreaction ,RT PCR)与蛋白质印迹 (Westernblot)方法测定脂肪肝肝组织中FATP4表达变化。
6)  Western blot
免疫印记
1.
Expression of HAPO in the liver was detected by SDS-PAGE and Western blot.
方法:杂交瘤方法制备抗HAPO的单克隆抗体;非竞争酶联免疫吸附实验测定抗体的相对亲和力;蛋白G亲和层析纯化腹水中的抗体,免疫印记方法检测胎肝中天然HAPO的表达。
参考词条
补充资料：blot hybridization
分子式：
CAS号：

性质：在基因操作中，把DNA或RNA、蛋白质等在薄膜滤器上先经浸润，固定后，于薄膜滤器上进行杂交，生成杂种分子。为基因操作中最常用的技术。常用的操作方法是：将用凝胶电泳分离出来的DNA或RNA、蛋白质等，在保持电泳图形的条件下，使其移至薄膜滤器上，再用事先准备好的检测样品(探针，经标记的某种基因等)进行杂交。以显示这些DNA、RNA、蛋白质等。从凝胶上转移DNA的技术称萨瑟恩(Southern)印迹技术。一般把转移RNA称为诺森(Northern)印迹技术，而把转移蛋白质称为韦斯顿(Western)印迹技术，当利用凝胶进行抗原抗体反应，再进行印迹时，称做伊斯特尔(Eastern)印迹技术。

说明：补充资料仅用于学习参考，请勿用于其它任何用途。