Fifteen target compounds were synthesized with the following three steps: amino acids were protected to form carbobenzoxyamino acid methyl esters, the carbobenzoxyamino acid methyl esters reacted with hydrazine to give carbobenzoxy amino acid hydrazides, and treatment of the carbobenzoxy amino acid hydrazides with glycosyl isothiocyanates afforded 3a～3o in 65%～86% yields.

The reaction of 2 with glycosyl isothiocyanates 3～5 gave the novel compounds, N-glycosyl-N'-amido thioureas 6～8.

The glycosyl isothiocyanates 1a～1c reacted with anhydrous hydrazine gave N-glycosyl- N'-aminothioureas 2a～2c, which then reacted further with 6-substituted-3-formylchromones 3a～3d to give a series of new N-glycosyl-N′-6-substitutedchromon-3-ylmethylideneaminothioureas 4a～4d, 5a～5d, 6a～ 6d.

999 3), that was well matched with the regression of methyl isothiocyanate, y=0.

A reversed-phase high performance liquid chromatographic method (HPLC) was established for determination of isothiocyanates in human urine, which used the reaction of propyl isothiocyanate with 1,2-benzenedithiol as reference instead of the synthesis of 1,3-benzodithiole-2-thione.