1) yeast capture vector
酵母捕捉载体
1.
The yeast-based capture system was employed to construct yeast capture vector YCV6 which is used for arg6 tRNA gene site and cloning the identified genomic islands in this site of Klebsiella pneumoniae clinical strains.
具体方法为:对20株从临床中分离的肺炎克雷伯菌进行体外tRIP PCR扩增以搜索外源基因组岛;利用酵母同源重组系统构建作用于arg6 tRNA基因位点的酵母捕捉载体YCV6并克隆该位点的基因组岛。
2) yeast carrier
酵母载体
1.
The utilization of stabilized active proliferous yeast carrier new techniques could square away the above disadvantages.
游离态间歇发酵工艺 ,操作程序复杂 ,生产效率低 ,应用固定化活性增殖酵母载体新技术能解决以上诸多难题。
3) stationary yeast carrying agent
固定化酵母载体
5) pPIC9K vector
pPIC9K酵母表达载体
1.
METHOD:A re comb inant expression plasmid pPIC 9K/hVEGF165 was constructed with pPIC9K vector and human VEGF 165 cDNA prepared from human tumor tissue,then transformed to yeas t host strain GS115 The positive transformants were screened out and cultured in flasks.
方法 :自人肿瘤组织中获取人VEGF1 65cDNA ,构建pPIC9K酵母表达载体 ,采用Pichia酵母真核表达系统表达目的蛋白。
6) yeast expression vector
酵母表达载体
1.
Construction of yeast expression vector and inducible expression of cDNA fragment of stilbene synthase in yeast;
葡萄芪合酶基因cDNA片段酵母表达载体的构建及其在酿酒酵母中的表达
2.
The yeast expression vectors construction of the barley yellow dwarf virus coat protein gene and the movement protein gene;
大麦黄矮病毒外壳蛋白基因和移动蛋白基因酵母表达载体的构建
3.
Through restrict endonuclase Sal I / Pst I,ligation,transformation,recombinant plasmids′ restrict identification and gene sequencing,yeast expression vectors pGBKT7-GPV-CP and pGBKT7-GPV-MP were constructed,used for expressing the bait fusion protein in yeast two-hybrid an.
根据已知的大麦黄矮病毒GPV株系的外壳蛋白(Coat Protein CP)和移动蛋白(Movement Protein MP)基因序列合成了CP、MP基因的上下游引物,通过PCR扩增获得含有目的基因的片段,经过Sal I和Pst I双酶切、连接、转化、重组质粒的酶切鉴定及基因测序,构建了酵母表达载体pGBKT7-GPV-CP和pGBKT7-GPV-MP,用于在酵母双杂交分析中表达诱饵融合蛋白,为进一步筛选小麦cDNA文库内与大麦黄矮病毒互作的寄主因子、克隆寄主因子、推测其种类和功能奠定了基础。
补充资料：贝克氏酵母菌