Development of the detetion of ELISA kit for Btcry2Ab/2Ac insecticidal protein in transgenic food;

Diversity of Bacillus thuringiensis insecticidal proteins;

Studies on Purification, Gene Cloning and Expression of Insecticidal Protein;

The levels of Cry1Ab protein expression in pollen of the two transgenic Bt corns were quantified by using enzyme-linked immunosorbent essay (ELISA).

【Method】The levels of Cry1Ab protein expression in two transgenic Bt corn seedling debris of materials MON810 and Bt11 were quantified by using enzyme-linked immunosorbent essay (ELISA).

To probe the pathogenic mechanism and provide theoretical basis for safety and sustainable utilization of Bt corn,the histopathological changes in the midgut of larvae of the Asian corn borer,Ostrinia furnacalis (Guenée),fed on Bt-transgenic corn expressing Cry1Ab protein were observed by transmission electron microscopy (TEM).

The cry1A gene sequence fragments and Bt insecticidal protein in these samples(collected after the last season tillage)were explored using PCR amplification and enzyme-linked immunosorbent assay(ELISA)method,respectively.

Transgenic Bt cotton NUCONT33~B and transgenic Cry1Ac/CpTI rice lines, MSA and MSB, containing a synthetic cry1Ac gene from Bacillus thuringiensis, were used to study the resistance and degradation dynamics of Bt insecticidal proteins in the tissues of Bt crops under different situations by ELISA.

So a rapid and accurate method is very necessary for detection of Bt insecticidal protein.

Tissue distribution and content of Cry1Ab insecticidal protein in the Bacillus thuringiensis resistant and susceptible Asian corn borer larvae fed on Bt-transgenic corn;

Biotransfer and bioaccumulation of Cry1Ab insecticidal protein in rice plant-brown planthopper-wolf spider food chain;

A laboratory study was made on the effects of transgenic corn expressing cry1Ab toxin from the bacterium Bacillus thuringiensis(Bt) on the life table of an experimental population of corn leaf aphid Rhopalosiphum maidis Fitch.