3) immunocytochemistry and immunofluorescence
免疫组化和免疫荧光
4) Immunofluorescence histochemistry
免疫荧光组织化学
1.
The dual immunofluorescence histochemistry showed that VGluT1- and VGluT2-like immunoreactive (-LI) axon terminals were in close apposition to the GFAP-LI structures, while VGluT3-LI axon terminals not only made close apposition but also coexpressed with the GFAP-LI structures in the spinal cord of the rat.
本研究应用双重免疫荧光组织化学法观察了大鼠脊髓胶质细胞内囊泡膜谷氨酸转运体 (VGlu Ts包括 VGlu T1,VG-lu T2及 VGlu T3 )的表达状况。
2.
The distribution of the neurofibromin, the NF1 gene product, in the adult rat brain was studied by using immunofluorescence histochemistry.
用免疫荧光组织化学方法观察了 NF1基因表达产物神经纤维素在正常成年大鼠脑中的分布 ,结果如下 :(1)大脑皮质中神经纤维素阳性细胞最为密集 ;其次 ,在海马结构、丘脑前核群、丘脑内侧核群、丘脑板内核群、丘脑中线核群及下丘脑室周核、室旁核中神经纤维素阳性细胞也较密集 ;在杏仁核、下丘脑前区、下丘脑外侧区、下丘脑后区以及隔外侧核、隔内侧核、尾壳核、屏状核、苍白球、三叉神经脊束核等部位 ,也可见到数量不等的神经纤维素阳性细胞稀疏分布。
3.
The control group and heat stress group mice were killed by cervical dislocation,when they were treated duration of heat stress to the day 8,13,21 and 35,and the percentage of abnormal sperm in the epididymis was detected by using the modified Papanicolaou staining method,and the expression of HSP70 in the epididymis detected by immunofluorescence histochemistry and western blot analysis methods.
在热应激持续到8、13、21和35天时,颈椎脱臼处死对照组和热应激组小鼠,应用改良巴氏染色法检测附睾精子畸形率,免疫荧光组织化学和蛋白质印迹分析的方法检测附睾组织HSP70的表达。
5) fluorescent immunohistochemistry
免疫荧光组织化学染色
6) immunofluorescence-histochemistry
免疫荧光组织化学
1.
The immunofluorescence-histochemistry and laser-confocal microscopy were used to observe the expression of NgR protein.
方法使用免疫荧光组织化学技术及激光共聚焦显微镜观察6只出生24h的正常SD大鼠视网膜中NgR蛋白的表达。
2.
The pathological changes in the early stage in the damaged area were observed by using Nissl staining;the expression of nNOS protein and its cells with positive reaction were examined with Western blot and immunofluorescence-histochemistry respectively.
于伤后0、1、3、6、12、24h等6个时间点分别采用尼氏染色法、免疫荧光组织化学方法、Western blot法来观察创伤区及周边皮质神经元的病理变化、nNOS阳性细胞和nNOS蛋白的表达变化。
补充资料：精子间接免疫荧光法
精子间接免疫荧光法
诊法。免疫学检查方法之 一。用于不育症检查。①以志愿供者的精液02ml加入10～12ml生理盐水中离心，取其精子沉淀部分再置入05ml生理盐水内。②取一滴作玻璃涂片，并在空气内晾干30分钟，用纯甲醇固定30分钟，再晾干后置入pH为74的磷酸缓冲液内15分钟。③被测血清滴于上述制备的玻片上，室温下孵化30～60分钟后，用磷酸缓冲液漂洗3次，每次10分钟。④玻片上滴注 附有抗人体免疫球蛋白抗血清的异硫氰酸荧光素盐酸盐，室温下孵化30～60分钟，孵化后用磷酸缓冲液漂洗3次，每次10分钟。⑤荧光显微镜下观察结果。利用血清内的精子抗体，可使精子不同部位呈现不同免疫荧光素反应，通过与正常血清对比研究，以判断精子抗体的存在情况。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条