2) integration and expression of foreign gene
整合与表达
1.
This paper reviews the new ways of the production of transgenic animals, the characteristics of integration and expression of foreign genes, and the several measures for increasing efficiency of gene expression in transgenic animals.
本文综述了近年来发展起来的转基因新方法及技术路线 ,转基因的整合与表达特点及提高转基因表达水平的策略
4) integrative expression
整合表达
6) replication and expression
复制与表达
1.
To explore possibility of hepatitis B virus(HBV)vertically transmitting via spermatozoon,to study the replication and expression of sperm-mediated HBV genes in two-cell embryos of golden hamster.
方法:将金黄地鼠精子与pBR322-HBVDNA重组质粒进行共培养后,与金黄地鼠正常卵母细胞受精,取2-细胞胚分别制片和提取RNA,用荧光原位杂交(Fluorescenceinsituhybridization,FISH)、RT-PCR和免疫荧光检测技术研究精子携带的HBV基因在胚胎细胞中的复制与表达。
2.
BACKGROUND&AIM: To explore the feasibility of hepatitis B virus(HBV)vertical transmission via male germ line,the replication and expression of sperm-mediated HBV DNA in mouse embryo were studied.
背景与目的:研究乙型肝炎病毒(HepatitisBvirus,HBV)DNA在早期胚胎中能否复制与表达,探讨HBV经雄性生殖细胞垂直传递的可能性。
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